限制性内切酶的DNA酶I足迹法
DNAase I footprinting of restriction enzymes.
作者信息
Fox K R
机构信息
Dept. of Physiology and Pharmacology University of Southampton, U.K.
出版信息
Biochem Biophys Res Commun. 1988 Sep 15;155(2):779-85. doi: 10.1016/s0006-291x(88)80563-1.
PMID:2844179
Abstract
DNAase I footprinting of restriction enzymes has been achieved by using calcium containing digestion buffers so that the enzymes bind to but do not cleave DNA. EcoR1 produces a footprint 17 bases long, overestimating the region of contact with DNA by about 7-8 base pairs. Restriction enzymes HaeIII and HinP1 generate smaller footprints of 15 and 13 base pairs respectively.
摘要
通过使用含钙的消化缓冲液实现了对限制酶的DNA酶I足迹分析，这样酶就能结合到DNA上但不会切割DNA。EcoR1产生一个17个碱基长的足迹，与DNA的接触区域估计高估了约7 - 8个碱基对。限制酶HaeIII和HinP1分别产生15个和13个碱基对的较小足迹。
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