米口袋化学成分及其抗炎活性。
Chemical constituents from Gueldenstaedtia verna and their anti-inflammatory activity.
作者信息
Liu Xin, Yin Chengle, Cao Yue, Zhou Jinge, Wu Tao, Cheng Zhihong
机构信息
a Department of Pharmacognosy , School of Pharmacy, Fudan University , Shanghai , China.
b Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
出版信息
Nat Prod Res. 2018 May;32(10):1145-1149. doi: 10.1080/14786419.2017.1320795. Epub 2017 Apr 25.
A new triterpenoid glycoside (1) and 15 known compounds (2-16) were isolated from the whole plants of Gueldenstaedtia verna. The new compound (1) was identified as complogenin 22-O-β-d-glucopyranoside by extensive spectroscopic techniques including 1D (H and C) and 2D NMR experiments (HSQC, HMBC and NOESY), HR-DART-MS and chemical methods. Most of the isolates were evaluated for their inhibitory activities on LPS-induced NO production in RAW 264.7 cells. The inhibitory effects of the active compounds, sulphuretin (8) and (22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol (13), on the production of pro-inflammatory mediators (including IL-6, IL1β and PGE) were further estimated in vitro by ELISA in RAW 264.7 macrophages.
从米口袋全草中分离得到一个新的三萜糖苷（1）和15个已知化合物（2 - 16）。通过包括一维（氢和碳）和二维核磁共振实验（HSQC、HMBC和NOESY）、高分辨直接分析实时质谱以及化学方法等广泛的光谱技术，将新化合物（1）鉴定为绵马酚元22 - O - β - D - 吡喃葡萄糖苷。对大多数分离物进行了它们对RAW 264.7细胞中脂多糖诱导的一氧化氮产生的抑制活性评估。通过酶联免疫吸附测定法在RAW 264.7巨噬细胞中进一步体外评估了活性化合物杨梅黄素（8）和（22E，24S）-5α，8α - 环氧 - 24 - 甲基 - 胆甾 - 6，9（11），22 - 三烯 - 3β - 醇（13）对促炎介质（包括白细胞介素 - 6、白细胞介素 - 1β和前列腺素E）产生的抑制作用。
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