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基于生物活性导向分离及核磁共振的豆科植物（L.）Desv. ex DC.根中免疫调节异黄酮的鉴定

Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of (L.) Desv. ex DC.

作者信息

Tu Ping-Chen, Chan Chih-Ju, Liu Yi-Chen, Kuo Yueh-Hsiung, Lin Ming-Kuem, Lee Meng-Shiou

机构信息

Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung 404, Taiwan.

Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan.

出版信息

Foods. 2019 Nov 3;8(11):543. doi: 10.3390/foods8110543.

DOI:10.3390/foods8110543
PMID:31684126
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6915426/
Abstract

is used as a functional food ingredient. Little is known about the association between its immunomodulatory activity and its metabolites. We applied a precise strategy for screening metabolites using immunomodulatory fractions from a root methanolic extract (UCME) in combination with bioactivity-guided fractionation and NMR-based identification. The fractions from UCME were evaluated in terms of their inhibitory activity against the production of pro-inflammatory cytokines (IL-6 and TNF-) by lipopolysaccharide (LPS)-stimulated mouse bone marrow-derived dendritic cells (BMDC). The role of the isoflavone genistein was indicated by the H NMR profiling of immunomodulatory subfractions (D-4 and D-5) and supported by the result that genistein-knockout subfractions (D-4 / and D-5 /) had a lower inhibitory activity compared to genistein-containing subfractions. This study suggests that genistein contributes to the immunomodulatory activity of UCME and will help in the standardization of functional food.

摘要

它被用作功能性食品成分。关于其免疫调节活性与其代谢产物之间的关联，人们所知甚少。我们采用了一种精确的策略，利用来自根部甲醇提取物（UCME）的免疫调节级分，结合生物活性导向分级分离和基于核磁共振的鉴定方法来筛选代谢产物。对UCME的级分进行了评估，考察它们对脂多糖（LPS）刺激的小鼠骨髓来源树突状细胞（BMDC）产生促炎细胞因子（IL-6和TNF-）的抑制活性。免疫调节亚级分（D-4和D-5）的氢核磁共振谱图表明了异黄酮染料木黄酮的作用，并且染料木黄酮基因敲除亚级分（D-4 /和D-5 /）与含染料木黄酮的亚级分相比具有较低的抑制活性，这一结果也支持了上述作用。本研究表明，染料木黄酮有助于UCME的免疫调节活性，并且将有助于功能性食品的标准化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/be77519bc100/foods-08-00543-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/406ab0f9e06f/foods-08-00543-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/9432c19e4942/foods-08-00543-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/422431ac5250/foods-08-00543-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/60664a09f0be/foods-08-00543-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/bf1cdce2d97f/foods-08-00543-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/796948d66bfa/foods-08-00543-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/be77519bc100/foods-08-00543-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/406ab0f9e06f/foods-08-00543-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/9432c19e4942/foods-08-00543-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/422431ac5250/foods-08-00543-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/60664a09f0be/foods-08-00543-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/bf1cdce2d97f/foods-08-00543-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/796948d66bfa/foods-08-00543-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d1/6915426/be77519bc100/foods-08-00543-g007.jpg

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2
Identification of antiplasmodial triterpenes from Keetia species using NMR-based metabolic profiling.采用基于 NMR 的代谢组学方法鉴定 Keetia 属植物中的抗疟三萜。
Metabolomics. 2019 Feb 21;15(3):27. doi: 10.1007/s11306-019-1487-4.
3
Two New Triterpene Glycosides in the Roots of Uraria crinita.毛鱼藤根中的两种新三萜糖苷。
现代方法在发现和开发植物源天然产物及其类似物作为潜在治疗药物中的应用。
Molecules. 2022 Jan 6;27(2):349. doi: 10.3390/molecules27020349.
4
Development of Food Chemistry, Natural Products, and Nutrition Research: Targeting New Frontiers.食品化学、天然产物与营养研究的发展：瞄准新前沿。
Foods. 2020 Apr 12;9(4):482. doi: 10.3390/foods9040482.
Chem Pharm Bull (Tokyo). 2019;67(2):159-162. doi: 10.1248/cpb.c18-00753.
4
Dietary Genistein Alleviates Lipid Metabolism Disorder and Inflammatory Response in Laying Hens With Fatty Liver Syndrome.膳食染料木黄酮可缓解脂肪肝综合征蛋鸡的脂质代谢紊乱和炎症反应。
Front Physiol. 2018 Oct 24;9:1493. doi: 10.3389/fphys.2018.01493. eCollection 2018.
5
Metabolomic approach for a rapid identification of natural products with cytotoxic activity against human colorectal cancer cells.代谢组学方法快速鉴定对人结肠直肠癌细胞具有细胞毒性的天然产物。
Sci Rep. 2018 Mar 28;8(1):5309. doi: 10.1038/s41598-018-23704-9.
6
Phenylpropionamides, Piperidine, and Phenolic Derivatives from the Fruit of Ailanthus altissima.臭椿果实中的苯丙酰胺类、哌啶类和酚类衍生物。
Molecules. 2017 Dec 4;22(12):2107. doi: 10.3390/molecules22122107.
7
Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics.基于核磁共振代谢组学的叶下珠代谢物谱特征分析及其与生物活性的相关性研究
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Nat Prod Res. 2017 Jan;31(2):131-137. doi: 10.1080/14786419.2016.1217206. Epub 2016 Aug 8.