Zhang Xiaolei, Chen Jiaxin, Yan Song, Tyagi Rajeshwar Dayal, Surampalli Rao Y, Li Ji
Water Environ Res. 2017 May 1;89(5):424-439. doi: 10.2175/106143017X14839994523424.
Oleaginous yeast Trichosporon oleaginosus was studied for lipid production using municipal sludge with or without fortification of crude glycerol in a 15-L fermenter. The maximum lipid content (concentration) was 32.0% w/w (9.35 g/L), 33.6% (10.13 g/L), 33.3% (9.13 g/L), and 33.1% (9.03 g/L) w/w with the addition of 25, 50, 100, and 150 g/L glycerol, respectively. Glycerol concentration had little effect on lipid accumulation. However, glycerol concentration substantially affected increase of biomass concentration and cell count. The suitable glycerol concentration was approximately 40 g/L for Trichosporon oleaginosus growing in sludge medium with initial suspended solids (SS) concentration 30 g/L. Addition of nitrogen to sludge-glycerol medium enhanced lipid and biomass concentration. The energy conversion efficiency was 1.78, 1.55, and 1.71 with no nitrogen added, with addition of 1 g/L urea, and 3.7 g/L peptone, respectively. The biodiesel production cost was estimated nearly 0.75 US$/L.
研究了产油酵母油脂性丝孢酵母在15升发酵罐中利用城市污泥生产脂质的情况，污泥中添加或不添加粗甘油。添加25、50、100和150克/升甘油时，最大脂质含量（浓度）分别为32.0%（重量/重量）（9.35克/升）、33.6%（10.13克/升）、33.3%（9.13克/升）和33.1%（9.03克/升）（重量/重量）。甘油浓度对脂质积累影响不大。然而，甘油浓度对生物量浓度和细胞数量的增加有显著影响。对于初始悬浮固体（SS）浓度为30克/升的污泥培养基中生长的油脂性丝孢酵母，合适的甘油浓度约为40克/升。向污泥 - 甘油培养基中添加氮可提高脂质和生物量浓度。不添加氮、添加1克/升尿素和3.7克/升蛋白胨时，能量转换效率分别为1.78、1.55和1.71。估计生物柴油生产成本接近0.75美元/升。
