以粗甘油为碳源，利用 pH 值控制的补料分批发酵法生产油脂的油脂酵母（Trichosporon oleaginosus）。
The pH-based fed-batch for lipid production from Trichosporon oleaginosus with crude glycerol.
机构信息
INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen 518055, PR China.
出版信息
Bioresour Technol. 2018 Jul;259:237-243. doi: 10.1016/j.biortech.2018.03.045. Epub 2018 Mar 13.
In this study, it was found that the optimal pH for the growth of Trichosporon oleaginosus was related to the fermentation medium. A neutral or weak acid pH condition was optimal for the growth of Trichosporon oleaginosus in the extract-peptone-dextrose and wastewater sludge medium. Significant inhibition was observed at neutral pH in the wastewater sludge + crude glycerol medium due to the high soap content of the crude glycerol. By converting the soap to free fatty acid (FFA) at pH 5, the soap inhibition could be prevented. Fed-batch fermentation was employed to produce lipid from Trichosporon oleaginosus at pH 5 controlled by feeding crude glycerol. A remarkably high biomass (65.63 g/L) and lipid (35.79 g/L) concentration were achieved from the pH-based fed-batch fermentation in this study.
在这项研究中发现，产油假丝酵母的最佳生长 pH 值与发酵培养基有关。在提取物-蛋白胨-葡萄糖和废水污泥培养基中，产油假丝酵母的最佳生长 pH 值为中性或弱酸性。在废水污泥+粗甘油培养基中，由于粗甘油中的肥皂含量很高，在中性 pH 值时会观察到明显的抑制作用。通过将肥皂在 pH 值为 5 时转化为游离脂肪酸（FFA），可以防止肥皂抑制。通过 fed-batch 发酵，在 pH 值为 5 时用粗甘油进料来生产产油假丝酵母的脂质。在这项研究中，通过基于 pH 值的 fed-batch 发酵，实现了非常高的生物量（65.63 g/L）和脂质（35.79 g/L）浓度。
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