INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, PR China.
Bioresour Technol. 2018 Apr;253:8-15. doi: 10.1016/j.biortech.2018.01.008. Epub 2018 Jan 3.
In this work, methanol in crude glycerol solution was used to assist the lipid production with oleaginous yeast Trichosporon oleaginosus cultivated under non-sterilized conditions. The investigated methanol concentration was 0%, 1.4%, 2.2%, 3.3% and 4.4% (w/v). The results showed that methanol played a significant role in the non-sterilized fermentation for lipid production. The optimal methanol concentration was around 1.4% (w/v) in which the growth of T. oleaginosus was promoted and overcame that of the contaminants. The non-sterilized fed-batch fermentation with initial methanol concentration of 1.4% (w/v) was then performed and high biomass production (43.39 g/L) and lipid production (20.42 g/L) were achieved.
在这项工作中，使用甲醇辅助在非灭菌条件下培养的产油酵母油脂酵母（Trichosporon oleaginosus）从粗甘油溶液中生产油脂。考察的甲醇浓度分别为 0%、1.4%、2.2%、3.3%和 4.4%（w/v）。结果表明，甲醇在非灭菌发酵生产油脂中起着重要作用。在最佳甲醇浓度约为 1.4%（w/v）时，促进了油脂酵母的生长，抑制了污染物的生长。然后进行初始甲醇浓度为 1.4%（w/v）的非灭菌分批补料发酵，实现了高生物量（43.39g/L）和油脂（20.42g/L）的生产。
