Lee Soohyun, Hwang Eunjin, Lee Dongmyeong, Choi Jee Hyun
Center for Neuroscience, Korea Institute of Science and Technology, Seoul 02792, Korea.
Department of Physics, Pohang University of Science and Technology, Pohang 37673, Korea.
Exp Neurobiol. 2017 Apr;26(2):90-96. doi: 10.5607/en.2017.26.2.90. Epub 2017 Apr 10.
Human studies of brain stimulation have demonstrated modulatory effects on the perception of pain. However, whether the primary somatosensory cortical activity is associated with antinociceptive responses remains unknown. Therefore, we examined the antinociceptive effects of neuronal activity evoked by optogenetic stimulation of primary somatosensory cortex. Optogenetic transgenic mice were subjected to continuous or pulse-train optogenetic stimulation of the primary somatosensory cortex at frequencies of 15, 30, and 40 Hz, during a tail clip test. Reaction time was measured using a digital high-speed video camera. Pulse-train optogenetic stimulation of primary somatosensory cortex showed a delayed pain response with respect to a tail clip, whereas no significant change in reaction time was observed with continuous stimulation. In response to the pulse-train stimulation, video monitoring and local field potential recording revealed associated paw movement and sensorimotor rhythms, respectively. Our results show that optogenetic stimulation of primary somatosensory cortex at beta and gamma frequencies blocks transmission of pain signals in tail clip test.
对人类大脑刺激的研究已证明其对疼痛感知具有调节作用。然而，初级体感皮层活动是否与抗伤害感受反应相关仍不清楚。因此，我们研究了通过光遗传学刺激初级体感皮层诱发的神经元活动的抗伤害感受作用。在一项夹尾试验中，对光遗传学转基因小鼠的初级体感皮层进行15、30和40赫兹频率的连续或脉冲序列光遗传学刺激。使用数字高速摄像机测量反应时间。初级体感皮层的脉冲序列光遗传学刺激显示相对于夹尾而言疼痛反应延迟，而连续刺激时未观察到反应时间有显著变化。响应脉冲序列刺激时，视频监测和局部场电位记录分别显示相关的爪部运动和感觉运动节律。我们的结果表明，在夹尾试验中，以β和γ频率对初级体感皮层进行光遗传学刺激可阻断疼痛信号的传递。
