Kaźmierczak Urszula, Banaś Dariusz, Braziewicz Janusz, Buraczewska Iwona, Czub Joanna, Jaskóła Marian, Kaźmierczak Łukasz, Korman Andrzej, Kruszewski Marcin, Lankoff Anna, Lisowska Halina, Nesteruk Marta, Szefliński Zygmunt, Wojewódzka Maria
Faculty of Physics, University of Warsaw, ul. Hoża 69, 00-681 Warsaw, Poland.
Institute of Physics, Jan Kochanowski University, ul. Świętokrzyska 15, 25-406 Kielce, Poland.
Rep Pract Oncol Radiother. 2014 May 19;19(Suppl):S37-S41. doi: 10.1016/j.rpor.2014.04.015. eCollection 2014 May.
Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1-4 Gy of high LET C ions and X-rays.
The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron therapy and space radiation biology.
A heavy ion C beam from the Heavy Ion Laboratory of the University of Warsaw (HIL) was used to irradiate CHO-K1 cells. Cells were seeded in Petri dishes specially designed for irradiation purposes. Immediately after irradiation, cells were transferred into transwell culture insert dishes to enable co-culture of irradiated and non-irradiated cells. Cells from the membrane and well shared the medium but could not touch each other. To study bystander effects, a clonogenic survival assay was performed.
The survival fraction of cells co-cultured with cells irradiated with C ions and X-rays was not reduced.
The bystander effect was not observed in these studies.
研究与受0.1 - 4 Gy高传能线密度碳离子和X射线照射的细胞共培养的中国仓鼠卵巢细胞（CHO-K1）中的旁观者效应。
带电重粒子在细胞或分子水平上的放射生物学效应在生物医学应用领域至关重要，尤其是在强子治疗和空间辐射生物学中。
使用华沙大学重离子实验室（HIL）的重离子碳束照射CHO-K1细胞。细胞接种在专门设计用于照射的培养皿中。照射后立即将细胞转移到Transwell共培养插入式培养皿中，以使照射和未照射的细胞能够共培养。膜上和孔中的细胞共享培养基，但彼此不能接触。为了研究旁观者效应，进行了克隆形成存活试验。
与受碳离子和X射线照射的细胞共培养的细胞的存活分数没有降低。
在这些研究中未观察到旁观者效应。
