用于测定食蟹猴（猕猴）肾小球滤过率的碘海醇清除率
Iohexol Clearance for Determination of Glomerular Filtration Rate in Cynomolgus Monkeys (Macaca fascicularis).
作者信息
Zhang Changqing, Ding Shanshan, Fang Yupeng, Zhang Li, Hu Weimin, Lu Jinlian, Jing Tao, Tao Yi, Zhang Xin
机构信息
Large Animal In Vivo Pharmacology Group, Lab Testing Division, Wuzhong District, Suzhou, Jiangsu Province, China; Email:
Large Animal In Vivo Pharmacology Group, Lab Testing Division, Wuzhong District, Suzhou, Jiangsu Province, China
出版信息
J Am Assoc Lab Anim Sci. 2017 May 1;56(3):330-333. Epub 2017 Apr 25.
The purpose of this study was to validate a method for determine the glomerular filtration rate (GFR) in healthy cynomolgus monkeys by using iohexol. Eighteen healthy cynomolgus macaque monkeys (age, 4 to 6 y [mean, 5 y]; weight, 2 to 6 kg [mean, 4 kg]) were randomly entered into 3 different doses groups (3 male and 3 female macaques per group) of 30, 60, 90 mg I/kg to receive an intravenous bolus injection of iohexol. Serum iohexol concentrations were determined by using liquid chromatography–tandem mass spectrometry, and clearance rate were determined by using WinNonlin software. The GFR value (mean ± SD) of each dose group was 2.50 ± 0.321, 2.65 ± 0.529, and 2.75 ± 0.385 mL/min/kg. These values did not differ significantly between dose levels or sexes. Iohexol clearance is a simple, precise method that is suitable for the determination of GFR in cynomolgus monkeys.
本研究的目的是验证一种通过使用碘海醇来测定健康食蟹猴肾小球滤过率（GFR）的方法。18只健康食蟹猕猴（年龄4至6岁[平均5岁]；体重2至6千克[平均4千克]）被随机分为3个不同剂量组（每组3只雄性和3只雌性猕猴），分别接受30、60、90毫克碘海醇/千克的静脉推注。血清碘海醇浓度采用液相色谱 - 串联质谱法测定，清除率使用WinNonlin软件测定。每个剂量组的GFR值（平均值±标准差）分别为2.50±0.321、2.65±0.529和2.75±0.385毫升/分钟/千克。这些值在剂量水平或性别之间无显著差异。碘海醇清除率是一种简单、精确的方法，适用于测定食蟹猴的GFR。
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