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坐骨神经受压后在水生环境中的跳跃:Wistar大鼠比目鱼肌的伤害性评估及形态学特征

Jumping in aquatic environment after sciatic nerve compression: nociceptive evaluation and morphological characteristics of the soleus muscle of Wistar rats.

作者信息

Malanotte Jéssica Aline, Kakihata Camila Mayumi Martin, Karvat Jhenifer, Brancalhão Rose Meire Costa, Ribeiro Lucinéia de Fátima Chasko, Bertolini Gladson Ricardo Flor

机构信息

Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil.

出版信息

Einstein (Sao Paulo). 2017 Jan-Mar;15(1):77-84. doi: 10.1590/S1679-45082017AO3613.

DOI:10.1590/S1679-45082017AO3613
PMID:28444094
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5433312/
Abstract

OBJECTIVE

To evaluate the effect of jumping in aquatic environment on nociception and in the soleus muscle of trained and not trained Wistar rats, in the treatment of compressive neuropathy of the sciatic nerve.

METHODS

Twenty-five Wistar rats were distributed into five groups: Control, Lesion, Trained + Lesion, Lesion + Exercise, and Trained + Lesion + Exercise. The training was jumping exercise in water environment for 20 days prior to injury, and treatment after the injury. Nociception was evaluated in two occasions, before injury and seven after injury. On the last day of the experiment, the right soleus muscles were collected, processed and analyzed as to morphology and morphometry.

RESULTS

In the assessment of nociception in the injury site, the Control Group had higher average than the rest, and the Lesion Group was larger than the Trained + Lesion and Lesion + Exercise Groups. The Control Group showed higher nociceptive threshold in paw, compared to the others. In the morphometric analysis, in relation to Control Group, all the injured groups showed decreased muscle fiber area, and in the Lesion Group was lower than in the Lesion + Exercise Group and Trained + Lesion Group. Considering the diameter of the muscle fiber, the Control Group had a higher average than the Trained + Lesion Group and the Trained + Lesion + Exercise Group; and the Lesion Group showed an average lower than the Trained + Lesion and Lesion + Exercise Groups.

CONCLUSION

Resistance exercise produced increased nociception. When performed prior or after nerve damage, it proved effective in avoiding hypotrophy. The combination of the two protocols led to decrease in diameter and area of the muscle fiber.

OBJETIVO

Avaliar os efeitos do salto em meio aquático, na nocicepção e no músculo sóleo, em ratos Wistar treinados e não treinados, no tratamento de neuropatia compressiva do nervo isquiático.

MÉTODOS: Foram distribuídos em cinco grupos 25 ratos Wistar: Controle, Lesão, Treinado + Lesão, Lesão + Exercício e Treinado + Lesão + Exercício. O treino foi com exercício de salto em meio aquático durante 20 dias, prévio à lesão, e o tratamento ocorreu após a lesão. Foram realizadas avaliações da nocicepção, sendo uma pré-lesão e sete pós-lesão. No último dia de experimento, os músculos sóleos direitos foram coletados, processados e analisados por meio de morfologia e morfometria.

RESULTADOS

Na avaliação da nocicepção no local da lesão, o Grupo Controle apresentou média maior que os demais, e o Grupo Lesão foi maior que os Grupos Treinado + Lesão e Lesão + Exercício. O Grupo Controle apresentou limiar nociceptivo na pata maior com relação aos demais. Nas análises morfométricas, em relação ao Grupo Controle, todos os grupos lesionados apresentaram diminuição da área da fibra muscular; o Grupo Lesão apresentou-se menor que os Grupos Treinado + Lesão e Lesão + Exercício. No diâmetro da fibra muscular, o Grupo Controle apresentou média maior que os Grupos Treinado + Lesão e Treinado + Lesão + Exercício, e o Grupo Lesão apresentou média menor que os Grupos Treinado + Lesão e Lesão + Exercício.

CONCLUSÃO: O exercício físico resistido produziu aumento da nocicepção. Quando realizado previamente ou após a lesão nervosa, mostrou-se eficaz em evitar a hipotrofia. A associação dos dois protocolos levou à diminuição do diâmetro e da área da fibra muscular.

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/f4d50c690b95/1679-4508-eins-15-01-0077-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/04bca981dc61/1679-4508-eins-15-01-0077-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/74053009f77a/1679-4508-eins-15-01-0077-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/5ab9eded091a/1679-4508-eins-15-01-0077-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/5a3b8b278363/1679-4508-eins-15-01-0077-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/5980a0190c93/1679-4508-eins-15-01-0077-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/e9bc30478e52/1679-4508-eins-15-01-0077-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/f4d50c690b95/1679-4508-eins-15-01-0077-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/04bca981dc61/1679-4508-eins-15-01-0077-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/74053009f77a/1679-4508-eins-15-01-0077-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/5ab9eded091a/1679-4508-eins-15-01-0077-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/5a3b8b278363/1679-4508-eins-15-01-0077-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/5980a0190c93/1679-4508-eins-15-01-0077-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/e9bc30478e52/1679-4508-eins-15-01-0077-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4bd/5433312/f4d50c690b95/1679-4508-eins-15-01-0077-gf07.jpg
摘要

目的

评估在水生环境中跳跃对训练和未训练的Wistar大鼠伤害感受及比目鱼肌的影响,用于坐骨神经压迫性神经病变的治疗。

方法

将25只Wistar大鼠分为五组:对照组、损伤组、训练+损伤组、损伤+运动组和训练+损伤+运动组。训练为在损伤前20天在水环境中进行跳跃运动,并在损伤后进行治疗。在损伤前和损伤后7天两个时间点评估伤害感受。在实验的最后一天,收集右侧比目鱼肌,进行处理并分析其形态和形态测量。

结果

在损伤部位的伤害感受评估中,对照组的平均值高于其他组,损伤组大于训练+损伤组和损伤+运动组。与其他组相比,对照组在爪部的伤害感受阈值更高。在形态测量分析中,与对照组相比,所有损伤组的肌纤维面积均减小,损伤组低于损伤+运动组和训练+损伤组。考虑到肌纤维直径,对照组的平均值高于训练+损伤组和训练+损伤+运动组;损伤组的平均值低于训练+损伤组和损伤+运动组。

结论

抗阻运动导致伤害感受增加。在神经损伤之前或之后进行时,它被证明可有效避免肌肉萎缩。两种方案的结合导致肌纤维直径和面积减小。

目的

评估在水生环境中跳跃对训练和未训练的Wistar大鼠伤害感受及比目鱼肌的影响,用于坐骨神经压迫性神经病变的治疗。

方法

将25只Wistar大鼠分为五组:对照组、损伤组、训练+损伤组、损伤+运动组和训练+损伤+运动组。训练为在损伤前20天在水环境中进行跳跃运动,并在损伤后进行治疗。在损伤前和损伤后7天两个时间点评估伤害感受。在实验的最后一天,收集右侧比目鱼肌,进行处理并分析其形态和形态测量。

结果

在损伤部位的伤害感受评估中,对照组的平均值高于其他组,损伤组大于训练+损伤组和损伤+运动组。与其他组相比,对照组在爪部的伤害感受阈值更高。在形态测量分析中,与对照组相比,所有损伤组的肌纤维面积均减小,损伤组低于损伤+运动组和训练+损伤组。考虑到肌纤维直径,对照组的平均值高于训练+损伤组和训练+损伤+运动组;损伤组的平均值低于训练+损伤组和损伤+运动组。

结论

抗阻运动导致伤害感受增加。在神经损伤之前或之后进行时,它被证明可有效避免肌肉萎缩。两种方案的结合导致肌纤维直径和面积减小。

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