Pentjuss A, Stalidzans E, Liepins J, Kokina A, Martynova J, Zikmanis P, Mozga I, Scherbaka R, Hartman H, Poolman M G, Fell D A, Vigants A
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.
Department of Biological and Medical Sciences, Oxford Brookes University, Headington, OX, OX3 0BP, UK.
J Ind Microbiol Biotechnol. 2017 Aug;44(8):1177-1190. doi: 10.1007/s10295-017-1946-8. Epub 2017 Apr 25.
The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism's potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.
非常规酵母马克斯克鲁维酵母是许多生物技术过程中新兴的工业生产菌株。在此，我们展示了一个经实验验证且质量和电荷平衡的中心代谢生物量关联化学计量模型的应用，该模型用于评估野生型和改良型马克斯克鲁维酵母的碳转化效率。通过各种建模和优化方法研究了底物对（乳糖、葡萄糖、菊粉、木糖）和产物对（乙醇、乙酸、乳酸、甘油、乙酸乙酯、琥珀酸、谷氨酸、苯乙醇和苯丙氨酸）。我们的模型揭示了该生物体在工业应用和代谢工程方面的潜力。建模结果表明，通气方式可作为优化马克斯克鲁维酵母产物产量和通量分布的工具。此外，重新平衡NADH和NADPH的利用可用于提高底物转化效率。木糖被确定为马克斯克鲁维酵母具有生物技术应用前景的底物。
