Chen Hanting, Fan Gang, He Yang
State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
3 Biotech. 2017 May;7(1):62. doi: 10.1007/s13205-017-0679-8. Epub 2017 Apr 25.
Coptis herbs, which are important herbal medicinal materials, are the dried rhizomes of various plants. In China's herbal market, drying herbs can severely influence morphological markers such as shape, color, and odor, which make consumers difficult to precisely identify the herbs and effectively evaluate the quality. Here, we present the phylogenetic analysis of four Coptis herbal medicinal materials based on internal transcribed spacer sequences. C. chinensis, C. omeiensis, C. deltoidea and C. teeta constituted a monophyletic group. In this group, C. omeiensis and C. deltoidea cluster together, and they form the sister group with C. teeta, and C. chinensis locate the outermost of C. omeiensis, C. deltoidea and C. teeta. At the same time, the HPLC-DAD method was applied to simultaneously analyze main alkaloids from seventeen different samples. On the basis of the robust phylogenetic topology, the parsimony reconstructions of six effective medicinal constituents were implemented to elucidate the evolutionary history in Coptis herbs, and thus evaluate the herbal quality. The results showed that C. chinensis had been considered to be the best herb, not only the high content in single constituent but also in total alkaloids. In addition, all the samples from seventeen habitats were considered as qualified herbs, and they could meet the requirements of national quality standards for Coptis herbs in China.
黄连类药材是重要的草药原料，为多种植物的干燥根茎。在中国草药市场，干燥处理会严重影响草药的形态学特征，如形状、颜色和气味，这使得消费者难以精确识别草药并有效评估其质量。在此，我们基于内转录间隔区序列对四种黄连类药材进行了系统发育分析。黄连、峨眉黄连、三角叶黄连和云南黄连构成一个单系类群。在这个类群中，峨眉黄连和三角叶黄连聚在一起，它们与云南黄连形成姐妹群，而黄连位于峨眉黄连、三角叶黄连和云南黄连的最外层。同时，采用高效液相色谱 - 二极管阵列检测法对17个不同样品中的主要生物碱进行了同步分析。基于稳健的系统发育拓扑结构，对六种有效药用成分进行简约性重建，以阐明黄连类药材的进化历史，从而评估草药质量。结果表明，黄连一直被认为是最佳药材，不仅单一成分含量高，总生物碱含量也高。此外，来自17个产地的所有样品均被视为合格药材，它们能够满足中国黄连类药材的国家质量标准要求。
