Li Dongmei, Zhou Lili, Wang Qingwei, He Yang
1College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137 China.
National Institute of Measurement and Testing Technology, Chengdu, 610021 China.
3 Biotech. 2018 Jun;8(6):285. doi: 10.1007/s13205-018-1300-5. Epub 2018 Jun 4.
herbs are important herbal medicinal materials. The bioactive composition, the quality and medicinal efficacy of these herbs, are determined significantly by their geo-authentic features. Among the effective components of these herbs are seven organic acids (quinic, acetic, formic, tartaric, malic, succinic, and oxalic acids). However, no quantitative data of these seven acids in these herbs are available. Therefore, we developed a method for simultaneous separation and determination of the seven organic acids in herbs using gradient ion chromatography (mobile phase and gradient were shown in Table 1). The seven acids were separated and determined in no more than 35 min. We found that the organic acid levels in was obviously higher than in and , in particular, the content of quinic acid in was about eight times than that in and . Furthermore, we analyzed the relationships between the contents of organic acids and clinical effects, and found that organic acids (content of total acids or content of quinic acid) could act as an reference ingredient for quality evaluation in herbs. Our studies would lay the foundation for effective quality evaluation of these herbs.
草药是重要的中药材。这些草药的生物活性成分、质量和药效，很大程度上由其道地性特征决定。这些草药的有效成分中包括七种有机酸（奎尼酸、乙酸、甲酸、酒石酸、苹果酸、琥珀酸和草酸）。然而，这些草药中这七种酸的定量数据尚无可用。因此，我们开发了一种使用梯度离子色谱法同时分离和测定草药中七种有机酸的方法（流动相和梯度见表1）。这七种酸在不超过35分钟内被分离和测定。我们发现某草药中的有机酸水平明显高于另两种草药，特别是该草药中奎尼酸的含量约为另两种草药的八倍。此外，我们分析了有机酸含量与临床疗效之间的关系，发现有机酸（总酸含量或奎尼酸含量）可作为该草药质量评价的参考成分。我们的研究将为这些草药的有效质量评价奠定基础。
