Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
Department of Chemistry, University of Rome, Tor Vergata, via della Ricerca Scientifica, 00133, Rome, Italy.
Angew Chem Int Ed Engl. 2017 Nov 27;56(48):15210-15233. doi: 10.1002/anie.201701868. Epub 2017 Nov 8.
Triplex nucleic acids have recently attracted interest as part of the rich "toolbox" of structures used to develop DNA-based nanostructures and materials. This Review addresses the use of DNA triplexes to assemble sensing platforms and molecular switches. Furthermore, the pH-induced, switchable assembly and dissociation of triplex-DNA-bridged nanostructures are presented. Specifically, the aggregation/deaggregation of nanoparticles, the reversible oligomerization of origami tiles and DNA circles, and the use of triplex DNA structures as functional units for the assembly of pH-responsive systems and materials are described. Examples include semiconductor-loaded DNA-stabilized microcapsules, DNA-functionalized dye-loaded metal-organic frameworks (MOFs), and the pH-induced release of the loads. Furthermore, the design of stimuli-responsive DNA-based hydrogels undergoing reversible pH-induced hydrogel-to-solution transitions using triplex nucleic acids is introduced, and the use of triplex DNA to assemble shape-memory hydrogels is discussed. An outlook for possible future applications of triplex nucleic acids is also provided.
三链体核酸最近作为用于开发基于 DNA 的纳米结构和材料的丰富“工具箱”的一部分引起了人们的兴趣。本综述介绍了使用 DNA 三链体组装传感平台和分子开关。此外，还介绍了 pH 诱导的、可切换的三链体 DNA 桥接纳米结构的组装和解离。具体来说，描述了纳米粒子的聚集/解聚集、折纸瓦片和 DNA 环的可逆聚合以及三链体 DNA 结构作为用于组装 pH 响应系统和材料的功能单元的用途。示例包括负载半导体的 DNA 稳定的微胶囊、负载染料的 DNA 功能化金属有机骨架 (MOF) 以及负载物的 pH 诱导释放。此外，还介绍了使用三链体核酸进行可逆 pH 诱导水凝胶到溶液转变的刺激响应 DNA 水凝胶的设计，并讨论了使用三链体 DNA 组装形状记忆水凝胶。还展望了三链体核酸可能的未来应用。
