Li Zhao-Yang, Lin Jia, Sun Feng, Li Hui, Xia Jun, Li Xue-Nan, Ge Jing, Zhang Cong, Li Jin-Long
College of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
Harbin Sport University, Harbin, P. R. China.
Oncotarget. 2017 Apr 11;8(15):24142-24153. doi: 10.18632/oncotarget.15903.
Transportation is inevitable in the poultry industry, and it can induce stress to chicks in varying degrees, such as mild discomfort, sometimes even death. However, the research about the effects of transport stress on the weight loss and heart injury of chicks is lacking. To elucidate the underlying mechanism of transport stress-induced effects, chicks were transported for 2h, 4h and 8h. The creatinine kinase (CK) activities, the ionic contents, the ATPases activities and the transcription of the ATPase associated subunits in chick heart were detected. The results showed that transport stress increased the weight loss and the CK activity, disturbed the ionic (K+, Ca2+, Mg2+) homeostasis and inhibited the ATPase (Na+-K+-ATPase, Ca2+-ATPase, Mg2+-ATPase and Ca2+-Mg2+-ATPase) activities, increased the ATP content and downregulated the gene expression levels of the ATPase associated subunits in heart. In conclusion, transport stress disturbed the ionic homeostasis via modulating ion transporting ATPases and the transcriptions of the associated subunits, and ultimately induced weight loss and heart injury in chicks.
在禽类养殖行业中，运输是不可避免的，它会在不同程度上给雏鸡造成应激，比如轻度不适，有时甚至导致死亡。然而，关于运输应激对雏鸡体重减轻和心脏损伤影响的研究尚属空白。为阐明运输应激诱导效应的潜在机制，将雏鸡运输2小时、4小时和8小时。检测雏鸡心脏中的肌酸激酶（CK）活性、离子含量、ATP酶活性以及ATP酶相关亚基的转录情况。结果显示，运输应激增加了体重减轻和CK活性，扰乱了离子（K +、Ca2 +、Mg2 +）稳态，抑制了ATP酶（Na + -K + -ATP酶、Ca2 + -ATP酶、Mg2 + -ATP酶和Ca2 + -Mg2 + -ATP酶）活性，增加了ATP含量，并下调了心脏中ATP酶相关亚基的基因表达水平。总之，运输应激通过调节离子转运ATP酶及其相关亚基的转录扰乱了离子稳态，最终导致雏鸡体重减轻和心脏损伤。
