Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
National Engineering Research Center for Gelatin-based TCM, Dong-E E-Jiao Co., Ltd, Liaocheng, Shandong Province, China.
J Vet Intern Med. 2021 Sep;35(5):2449-2457. doi: 10.1111/jvim.16235. Epub 2021 Jul 31.
With the development of large-scale donkey farming in China, long-distance transportation has become common practice, and the incidence of intestinal diseases after transportation has increased. The intestinal microbiota is important in health and disease, and whether or not transportation disturbs the intestinal microbiota in donkeys has not been investigated.
To determine the effects of transportation on the fecal microbiota of healthy donkeys using 16S rRNA sequencing.
Fecal and blood samples were collected from 12 Dezhou donkeys before and after transportation.
Prospective controlled study. Cortisol, ACTH, and heat-shock protein 90 (HSP90) concentrations were measured. Sequencing of 16S rRNA was used to assess the microbial composition. Alpha diversity and beta diversity were assessed.
Results showed significant (P < .05) increases in cortisol (58.1 ± 14.6 to 71.1 ± 9.60 ng/mL), ACTH (163.8 ± 31.9 to 315.8 ± 27.9 pg/mL), and HSP90 (10.8 ± 1.67 to 14.6 ± 1.75 ng/mL) on the day of arrival. A significantly lower (P = .04) level of bacterial richness was found in fecal samples after transportation, compared with that before transportation without distinct changes in diversity. Most notably, donkeys had significant decreases in Atopostipes, Eubacterium, Streptococcus, and Coriobacteriaceae.
Transportation can induce stress in healthy donkeys and have some effect on the composition of the in fecal microbiota. Additional studies are required to understand the potential effect of these microbiota changes, especially significantly decreased bacteria, on the development intestinal diseases in donkeys during recovery from transportation.
随着中国大规模养驴业的发展，长途运输已成为常见做法，运输后的肠道疾病发病率有所增加。肠道微生物群在健康和疾病中很重要，运输是否会扰乱驴的肠道微生物群尚未得到研究。
通过 16S rRNA 测序确定运输对健康驴粪便微生物群的影响。
运输前后采集 12 匹德州驴的粪便和血液样本。
前瞻性对照研究。测量皮质醇、促肾上腺皮质激素 (ACTH) 和热休克蛋白 90 (HSP90) 浓度。使用 16S rRNA 测序评估微生物组成。评估 alpha 多样性和 beta 多样性。
结果显示，皮质醇（58.1±14.6 至 71.1±9.60ng/mL）、ACTH（163.8±31.9 至 315.8±27.9pg/mL）和 HSP90（10.8±1.67 至 14.6±1.75ng/mL）在到达当天显著增加（P<.05）。与运输前相比，运输后粪便样本中的细菌丰富度显著降低（P=.04），但多样性没有明显变化。值得注意的是，驴的 Atopostipes、Eubacterium、Streptococcus 和 Coriobacteriaceae 显著减少。
运输会引起健康驴的应激，对粪便微生物群的组成有一定影响。需要进一步研究以了解这些微生物变化的潜在影响，特别是明显减少的细菌，对运输后恢复期间驴肠道疾病的发展的影响。
