Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 06466 Seeland, Germany.
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
Sci Data. 2017 Apr 27;4:170044. doi: 10.1038/sdata.2017.44.
Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. 'Morex' was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).
大麦（Hordeum vulgare L.）是一种主要用作动物饲料和麦芽制造工业原料的谷类草。国际大麦基因组测序联盟（IBSC）利用层级式散弹枪测序，构建了大麦 cv. 'Morex' 的基于图谱的参考基因组序列。在这里，我们报告了实验和计算程序，用于（i）沿着全基因组物理图谱的最小平铺路径对超过 80,000 个细菌人工染色体（BAC）克隆进行测序和组装，（ii）找到并验证相邻 BAC 之间的重叠，（iii）构建 4,265 个非冗余序列支架，代表重叠 BAC 簇，以及（iv）使用高密度遗传图谱、光学图谱和染色体构象捕获测序（Hi-C）提供的位置信息，沿着大麦的七个染色体对这些 BAC 簇进行排序和定向。通过大麦基因组浏览器（BARLEX）可以综合访问这些序列和图谱资源。
