罗汉果粗提物中对PGC-1α启动子活性有中等影响的葫芦烷型糖苷。
Cucurbitane Glucosides from the Crude Extract of Siraitia grosvenorii with Moderate Effects on PGC-1α Promoter Activity.
作者信息
Niu Biao, Ke Chang-Qiang, Li Bo-Han, Li Yuanyuan, Yi Yongji, Luo Yongwei, Shuai Lin, Yao Sheng, Lin Li-Gen, Li Jia, Ye Yang
机构信息
State Key Laboratory of Drug Research and Natural Products Chemistry Department, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , 555 Zu-Chong-Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, People's Republic of China.
School of Life Science and Technology, ShanghaiTech University , Shanghai 201210, People's Republic of China.
出版信息
J Nat Prod. 2017 May 26;80(5):1428-1435. doi: 10.1021/acs.jnatprod.6b01086. Epub 2017 Apr 27.
Seven new cucurbitane glucosides, 11-oxomogrosides III E and IV (1 and 2), 11-oxoisomogroside V (3), 7-oxomogrosides III E and IV (4 and 5), and mogrosides VI A and VI B (6 and 7), were separated from the crude extract of Siraitia grosvenorii. The new structures were defined by analysis of their H and C NMR, 2D NMR, and HRESIMS data. Especially, the band-selective constant time HSQC and band-selective constant time HMBC techniques were recuited to elucidate the structures of the complex glucoside moieties. Using the PGC-1α promoter driven luciferase reporter assay, the isolated compounds were examined for PGC-1α promoter activity.
从罗汉果粗提物中分离出7种新的葫芦烷型糖苷，即11-氧代罗汉果苷III E和IV（1和2）、11-氧代异罗汉果苷V（3）、7-氧代罗汉果苷III E和IV（4和5）以及罗汉果苷VI A和VI B（6和7）。通过对其氢核磁共振（H NMR）、碳核磁共振（C NMR）、二维核磁共振（2D NMR）和高分辨电喷雾电离质谱（HRESIMS）数据的分析确定了这些新结构。特别是，采用带选择性恒时异核单量子相干（band-selective constant time HSQC）和带选择性恒时异核多键相关（band-selective constant time HMBC）技术来阐明复杂糖苷部分的结构。使用过氧化物酶体增殖物激活受体γ共激活因子1α（PGC-1α）启动子驱动的荧光素酶报告基因测定法，对分离得到的化合物进行了PGC-1α启动子活性检测。
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