Loschmidt Laboratories, Department of Experimental Biology, Masaryk University, Brno, Czech Republic.
Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic.
Nucleic Acids Res. 2017 Jul 3;45(W1):W393-W399. doi: 10.1093/nar/gkx285.
There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiple-point thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at http://loschmidt.chemi.muni.cz/fireprot.
人们一直致力于提高蛋白质的稳定性，以增强其在众多生物医学和生物技术应用中的可用性。最近已经开发出许多用于预测突变对蛋白质稳定性影响的计算工具。然而，现有工具通常只能预测对蛋白质稳定性影响较小的单点突变，并且还需要进行繁琐的蛋白质表达、纯化和表征。在这里，我们介绍 FireProt，这是一个用于自动设计多点热稳定突变体蛋白质的网络服务器，它在其计算核心中结合了结构和进化信息。FireProt 利用了十六种工具和三种蛋白质工程策略来进行可靠的蛋白质设计。该服务器配备了交互式、易于使用的界面，允许用户直接分析和可选地修改设计的热稳定突变体。FireProt 可免费在 http://loschmidt.chemi.muni.cz/fireprot 获取。
