Jo Sung Jun, Kim Jeung Won, Choi Hye Ok, Kim Jung Hwan, Kim Hyung Joong, Woo Sun Hee, Han Byung Hoon
Life Science Research Institute, E.S. Biotech. Co. Ltd., Cheonan 31257, Republic of Korea.
Department of Agronomia, Chungbuk National University, Cheongju 28644, Republic of Korea.
Biomol Ther (Seoul). 2017 May 1;25(3):329-336. doi: 10.4062/biomolther.2017.048.
Adipogenesis in murine preadipocyte 3T3L-1 has been used as a model system to study anti-obese bioactive molecules. During adipogenesis in 3T3-L1 preadipocytes, we found that capsanthin inhibited adipogenesis (IC₅₀; 2.5 μM) and also showed lipolytic activity in differentiated adipocytes from the preadipocytes (ED₅₀; 872 nM). We identified that the pharmacological activity of capsanthin on adipogenesis in 3T3-L1 was mainly due to its adrenoceptor-β₂-agonistic activity. In high-fat diet animal model study, capsanthin significantly enhanced spontaneous locomotive activities together with progressive weight-loss. The capsanthin-induced activation of kinetic behavior in mice was associated with the excessive production of ATP initiated by both the enhanced lipolytic activity together with accelerated oxidation of fatty acids due to the adrenoceptor β₂-agonistic activity of capsanthin. Capsanthin also dose-dependently increased adiponectin and p-AMPK activity in high fat diet animals, suggesting that capsanthin has both anti-obesity and insulin sensitizing activities.
小鼠前脂肪细胞3T3L - 1中的脂肪生成已被用作研究抗肥胖生物活性分子的模型系统。在3T3 - L1前脂肪细胞的脂肪生成过程中，我们发现辣椒红素抑制脂肪生成（IC₅₀；2.5 μM），并且在来自前脂肪细胞的分化脂肪细胞中也表现出脂解活性（ED₅₀；872 nM）。我们确定辣椒红素对3T3 - L1脂肪生成的药理活性主要归因于其肾上腺素能受体β₂激动活性。在高脂饮食动物模型研究中，辣椒红素显著增强自发运动活动并伴有渐进性体重减轻。辣椒红素诱导的小鼠动力行为激活与因辣椒红素的肾上腺素能受体β₂激动活性导致的脂解活性增强以及脂肪酸氧化加速共同引发的ATP过量产生有关。辣椒红素还能使高脂饮食动物体内的脂联素和p - AMPK活性呈剂量依赖性增加，这表明辣椒红素具有抗肥胖和胰岛素增敏活性。
