Liu Zhen, Hu Guo-Dong, Luo Xiao-Bo, Yin Bin, Shu Bin, Guan Jing-Zhi, Jia Chi-Yu
Department of Burns and Plastic Surgery, The 309th Hospital of PLA, Beijing 100091, P.R. China.
Department of Orthopedics, The 309th Hospital of PLA, Beijing 100091, P.R. China.
Biomed Rep. 2017 Mar;6(3):279-284. doi: 10.3892/br.2017.842. Epub 2017 Jan 13.
The aim of the present study was to evaluate the anti-aging effects of bone marrow-mesenchymal stem cells (BM-MSCs) in a D-galactose-induced skin aging rat model. Male Sprague Dawley rats were randomly divided into four groups (n=10/group) as follows: Normal control group; skin aging model group; MSC-treated group by subcutaneous multi-point injection. The skin aging model was established by a daily subcutaneous injection of 15% D-galactose (1,000 mg/kg) for 8 weeks. Rats in the MSC-treated groups were administered 3×10/ml BM-MSCs/green fluorescent protein (GFP) for 4 weeks, administered once per week. Oxidative/antioxidative parameters were evaluated, and morphological and ultrastructure analyses were performed. Rats in the model group exhibited the typical changes of aging skin. Compared with the control group, rats in the model group had significantly increased malondialdehyde (MDA) content (P<0.01), and decreased serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities (P<0.05). MSC treatment markedly ameliorated aging-induced oxidative stress in the skin. Histologically, rats in the model group exhibited loosely arranged epidermal cell layers and disorganized collagen fibers. BM-MSC treatment significantly improved the histological abnormalities, which was similar to those in the control group. In addition, 7 days after the final cell transplantation, GFP-positive cells were observed by fluorescence microscopy to be distributed in the dermis. Injection of BM-MSCs significantly improved the D-galactose-induced histological abnormalities of the skin, by promoting an antioxidant response and ameliorating oxidative stress in aged skin. Thus, BM-MSCs may be beneficial in the rejuvenation of aged skin.
本研究的目的是在D-半乳糖诱导的皮肤衰老大鼠模型中评估骨髓间充质干细胞（BM-MSCs）的抗衰老作用。雄性Sprague Dawley大鼠被随机分为四组（每组n = 10），如下：正常对照组；皮肤衰老模型组；通过皮下多点注射进行MSC治疗的组。通过每天皮下注射15% D-半乳糖（1000 mg/kg）持续8周建立皮肤衰老模型。MSC治疗组的大鼠每周给药一次，连续4周，给予3×10/ml BM-MSCs/绿色荧光蛋白（GFP）。评估氧化/抗氧化参数，并进行形态学和超微结构分析。模型组大鼠表现出典型的衰老皮肤变化。与对照组相比，模型组大鼠丙二醛（MDA）含量显著增加（P<0.01），血清超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性降低（P<0.05）。MSC治疗显著改善了衰老诱导的皮肤氧化应激。组织学上，模型组大鼠表现为表皮细胞层排列疏松和胶原纤维紊乱。BM-MSC治疗显著改善了组织学异常，与对照组相似。此外，在最后一次细胞移植7天后，通过荧光显微镜观察到GFP阳性细胞分布在真皮中。注射BM-MSCs通过促进抗氧化反应和改善老年皮肤的氧化应激，显著改善了D-半乳糖诱导的皮肤组织学异常。因此，BM-MSCs可能有助于老年皮肤的年轻化。
