Wong Edith D
Department of Genetics, Stanford University, 3165 Porter Drive, Stanford, CA, 94305, USA.
Methods Mol Biol. 2017;1611:169-182. doi: 10.1007/978-1-4939-7015-5_13.
Elucidating the function of individual proteins will help to create a comprehensive picture of cell biology, as well as shed light on human disease mechanisms, possible treatments, and cures. Due to its compact genome, and extensive history of experimentation and annotation, the budding yeast Saccharomyces cerevisiae is an ideal model organism in which to determine protein function. This information can then be leveraged to infer functions of human homologs. Despite the large amount of research and biological data about S. cerevisiae, many proteins' functions remain unknown. Here, we explore ways to use the Saccharomyces Genome Database (SGD; http://www.yeastgenome.org ) to predict the function of proteins and gain insight into their roles in various cellular processes.
阐明单个蛋白质的功能将有助于构建细胞生物学的全貌，同时也能揭示人类疾病机制、可能的治疗方法和治愈手段。由于其基因组紧凑，且有广泛的实验和注释历史，芽殖酵母酿酒酵母是确定蛋白质功能的理想模式生物。然后可以利用这些信息来推断人类同源物的功能。尽管关于酿酒酵母已有大量研究和生物学数据，但许多蛋白质的功能仍然未知。在这里，我们探索利用酵母基因组数据库（SGD；http://www.yeastgenome.org ）来预测蛋白质功能并深入了解其在各种细胞过程中的作用的方法。
