Manikandan Velu, Velmurugan Palanivel, Park Jung-Hee, Chang Woo-Suk, Park Yool-Jin, Jayanthi Palaniyappan, Cho Min, Oh Byung-Taek
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 54596, South Korea.
Department of Environmental Science, Periyar University, Salem, 636011, Tamil Nadu, India.
3 Biotech. 2017 May;7(1):72. doi: 10.1007/s13205-017-0670-4. Epub 2017 Apr 27.
In the present study, the use of silver oxide nanoparticles (AgO NPs) synthesized using Ficus benghalensis prop root extract (FBPRE) as a reducing and stabilizing agent is reported and evaluated for its antibacterial activity against dental bacterial strains. The effects of pH, extract concentration, metal ion concentration, and contact time were studied to confirm the optimum production of AgO NPs. Our results suggest that, by increasing the extract concentration and the time frame, there will be a significant increase in the formation of nanoparticles. The UV-vis adsorption spectra show the absorbance peak in the range of 430 nm, and FTIR spectral peaks indicate that the phytochemicals in the extract are responsible for the formation of the nanoparticles. The HR-TEM image, SAED, and XRD pattern confirmed the morphology (spherical), silver oxide 42.7 nm and silver 51.4 nm, and crystalline nature of the obtained nanoparticles, respectively. The blend of FBPRE and AgO NPs showed excellent antibacterial activities against the two-dental bacteria Streptococcus mutans and Lactobacilli sp. The study results suggest that the blend of synthesized AgO NPS and FBPRE will be useful in tooth paste as a germicidal agent after extensive investigation with animal models.
在本研究中，报告了使用孟加拉榕气生根提取物（FBPRE）作为还原剂和稳定剂合成的氧化银纳米颗粒（AgO NPs），并评估了其对牙科细菌菌株的抗菌活性。研究了pH值、提取物浓度、金属离子浓度和接触时间的影响，以确定AgO NPs的最佳产量。我们的结果表明，通过提高提取物浓度和延长时间范围，纳米颗粒的形成将显著增加。紫外可见吸收光谱显示在430 nm范围内有吸收峰，傅里叶变换红外光谱峰表明提取物中的植物化学物质是纳米颗粒形成的原因。高分辨率透射电子显微镜图像、选区电子衍射和X射线衍射图谱分别证实了所得纳米颗粒的形态（球形）、氧化银粒径为42.7 nm和银粒径为51.4 nm以及晶体性质。FBPRE和AgO NPs的混合物对两种牙科细菌变形链球菌和乳酸杆菌表现出优异的抗菌活性。研究结果表明，经过动物模型的广泛研究后，合成的AgO NPS和FBPRE的混合物作为杀菌剂在牙膏中将会有用。
