Mizan Md Furkanur Rahaman, Bang Hyeon-Jo, Sadekuzzaman Mohammad, Lee Nari, Kim Tae-Jo, Ha Sang-Do
a Department of Food Science and Technology, Advanced Food Safety Research group, Brain Korea 21 Plus , Chung-Ang University , Anseong , Republic of Korea.
b Food Safety Research Group , Korea Food Research Institute , Seongnam-si , Republic of Korea.
Biofouling. 2017 May;33(5):369-378. doi: 10.1080/08927014.2017.1316840. Epub 2017 Apr 28.
Vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. In this study, an UltraFast LabChip Real-Time PCR assay was evaluated for rapid detection and quantification of pathogenic V. parahaemolyticus isolates. Escherichia coli and Vibrio harveyi were used as negative controls. Twenty-six tdh-positive, biofilm-producing V. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). REP-PCR analysis showed that the majority of the V. parahaemolyticus isolates originated from seafood and that clinical specimens formed two major clusters at 92.8% and 32% similarity levels. The presence and quantification of Autoinducer-2 was carried out using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) after derivatization of Autoinducer-2 with 2, 3-diaminonaphthalene. The presence of tdh-positive V. parahaemolyticus in marine samples highlights the need for constant environmental monitoring to protect public health.
副溶血性弧菌是海洋和河口环境中的一种微生物，可导致人类因食用受污染的海鲜而患肠胃炎。在本研究中，评估了一种超快速实验室芯片实时聚合酶链反应（PCR）检测方法，用于快速检测和定量致病性副溶血性弧菌分离株。大肠杆菌和哈维氏弧菌用作阴性对照。通过重复外显子回文聚合酶链反应（REP-PCR）分析了26株tdh阳性、产生物膜的副溶血性弧菌分离株。REP-PCR分析表明，大多数副溶血性弧菌分离株源自海鲜，临床标本在相似度为92.8%和32%时形成两个主要聚类。在用2,3-二氨基萘对自诱导物-2进行衍生化后，使用高效液相色谱-荧光检测（HPLC-FLD）对自诱导物-2进行检测和定量。海洋样本中tdh阳性副溶血性弧菌的存在凸显了持续进行环境监测以保护公众健康的必要性。
