Ashrafudoulla Md, Mizan Md Furkanur Rahaman, Park Heedae, Byun Kye-Hwan, Lee Nari, Park Si Hong, Ha Sang-Do
Department of Food Science and Technology, Advanced Food Safety Research Group, Brain Korea 21 Plus, Chung-Ang University, Anseong, South Korea.
Food Safety Research Group, Korea Food Research Institute, Seongnam, South Korea.
Front Microbiol. 2019 Mar 20;10:513. doi: 10.3389/fmicb.2019.00513. eCollection 2019.
The objective of this study was to investigate the virulence factors, genetic relationship, antibiotic resistance profile and the biofilm formation ability of isolates on shrimp and mussel surfaces at 30°C. In this study, eight ( = 8) isolated from mussel were examined. We used the polymerase chain reaction (PCR) to examine the distribution of different genes, and Repetitive Extragenic Palindromic-PCR (REP-PCR) to compare the genetic relationship. Disk diffusion technique was used to assess antibiotic and multiple-antibiotic resistance. The biofilm formation assay, and field emission scanning electron microscopy (FE-SEM) were used to evaluate biofilm formation ability. Transmission Electron Microscope (TEM) was used to observe the morphological structure of bacterial cell. Our results indicated that the biofilm-associated genes, 16S rRNA, , and , were present in all the tested isolates ( = 8). Approximately, 62.5% (5 isolates among 8 isolates) isolates showed strong multiple-antibiotic resistance index with an average value of 0.56. All isolates ( = 8) showed strong genetic relationship and significant biofilm formation ability on shrimp and mussel surfaces. This study demonstrated that the presence of virulence factors, high multiple antibiotic resistance index (MARI) values, and effective biofilm formation ability of isolates could be a great threat to human health and economic values in future. It was also suggested that a high resistance rate to antibiotic could be ineffective for treating infections. The continuous monitoring of antibiotic, molecular and biofilm characteristics is needed to increase seafood safety.
本研究的目的是调查在30°C下虾和贻贝表面分离株的毒力因子、遗传关系、抗生素耐药谱以及生物膜形成能力。在本研究中，对从贻贝中分离出的八株（n = 8）菌株进行了检测。我们使用聚合酶链反应（PCR）检测不同基因的分布，并使用重复外显子回文PCR（REP-PCR）比较遗传关系。采用纸片扩散法评估抗生素和多重抗生素耐药性。使用生物膜形成试验和场发射扫描电子显微镜（FE-SEM）评估生物膜形成能力。使用透射电子显微镜（TEM）观察细菌细胞的形态结构。我们的结果表明，所有测试的菌株（n = 8）中均存在与生物膜相关的基因、16S rRNA、[此处原文缺失相关基因名称]和[此处原文缺失相关基因名称]。大约62.5%（8株中的5株）的菌株显示出较强的多重抗生素耐药指数，平均值为0.56。所有菌株（n = 8）在虾和贻贝表面均显示出较强的遗传关系和显著的生物膜形成能力。本研究表明，菌株毒力因子的存在、高多重抗生素耐药指数（MARI）值以及有效的生物膜形成能力可能在未来对人类健康和经济价值构成巨大威胁。还表明对抗生素的高耐药率可能对治疗[此处原文缺失相关细菌名称]感染无效。需要持续监测[此处原文缺失相关细菌名称]的抗生素、分子和生物膜特征，以提高海鲜安全性。
