Todorčević Marijana, Hilton Catriona, McNeil Catriona, Christodoulides Constantinos, Hodson Leanne, Karpe Fredrik, Pinnick Katherine E
a Oxford Centre for Diabetes, Endocrinology and Metabolism, Radcliffe Department of Medicine, University of Oxford , Oxford , UK.
b NIHR Oxford Biomedical Research Centre, OUH Trust, Churchill Hospital , Oxford , UK.
Adipocyte. 2017 Jan 2;6(1):40-55. doi: 10.1080/21623945.2016.1277052. Epub 2017 Jan 6.
Upper-body adiposity is associated with increased metabolic disease risk, while lower-body adiposity is paradoxically protective. Efforts to understand the underlying mechanisms require appropriate and reproducible in vitro culture models. We have therefore generated immortalised () human preadipocyte (PAD) cell lines derived from paired subcutaneous abdominal and gluteal adipose tissue. These cell lines, denoted APAD and GPAD display enhanced proliferation and robust adipogenic capacities. Differentiated APAD and GPAD adipocytes synthesize triglycerides de novo and respond lipolytically to catecholamine-stimulation. Importantly the cells retain their depot-of-origin 'memory' as reflected by inherent differences in fatty acid metabolism and expression of depot-specific developmental genes. These features make these cell lines an invaluable tool for the in vitro investigation of depot-specific human adipocyte biology.
上身肥胖与代谢疾病风险增加相关，而下身肥胖却具有出人意料的保护作用。要了解其潜在机制，需要合适且可重复的体外培养模型。因此，我们从配对的腹部皮下和臀部脂肪组织中生成了永生化的人前脂肪细胞（PAD）系。这些细胞系，即APAD和GPAD，表现出增强的增殖能力和强大的成脂能力。分化后的APAD和GPAD脂肪细胞能重新合成甘油三酯，并对儿茶酚胺刺激产生脂解反应。重要的是，这些细胞保留了其起源部位的“记忆”，这体现在脂肪酸代谢的固有差异和特定部位发育基因的表达上。这些特性使这些细胞系成为体外研究特定部位人类脂肪细胞生物学的宝贵工具。
