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间充质干细胞条件培养基通过调节胰岛素受体和人表皮生长因子受体3的磷酸化来促进MDA-MB-231细胞迁移并抑制A549细胞迁移。

Mesenchymal stem cell-conditioned medium promotes MDA-MB-231 cell migration and inhibits A549 cell migration by regulating insulin receptor and human epidermal growth factor receptor 3 phosphorylation.

作者信息

Li Pengfei, Zhou Hongwei, Di Guohu, Liu Jin, Liu Yang, Wang Zhihong, Sun Yinxuan, Duan Haifeng, Sun Junzhong

机构信息

Department of Oncology, Liaoning Medical College, Jinzhou, Liaoning 121000, P.R. China.

Department of Hematology and Oncology, The First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100039, P.R. China.

出版信息

Oncol Lett. 2017 Mar;13(3):1581-1586. doi: 10.3892/ol.2017.5641. Epub 2017 Jan 25.

DOI:10.3892/ol.2017.5641
PMID:28454294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5403177/
Abstract

Various and studies have linked mesenchymal stem cells (MSCs) with cancer, but little is known about the effect of MSCs on tumor progression. The present study aimed to analyze the role of the MSCs from different tissues, consisting of human bone marrow, adipose and the umbilical cord tissues, and the heterogeneity of tumors in tumor progression. By collecting the culture supernatants of MSCs as MSC-conditioned media (CMs), the present study found that MSC-CM produces no significant effect on the proliferation of MDA-MB-231 and A549 tumor cells. The migration of MDA-MB-231 cells was enhanced upon incubation with MSC-CM, while that of A549 cells was inhibited. Furthermore, the phosphorylation of insulin receptors (IRs) was upregulated in MSC-CM-treated MDA-MB-231 cells, while in MSC-CM-treated A549 cells, the phosphorylation of human epidermal growth factor receptor 3 (Her3) was downregulated. Taken together, the findings suggest that the phosphorylation of IR and Her3 may contribute to the discrepant effects of MSC-CM on the migration of the 2 cell lines.

摘要

各种研究已将间充质干细胞（MSCs）与癌症联系起来，但关于MSCs对肿瘤进展的影响却知之甚少。本研究旨在分析源自人骨髓、脂肪和脐带组织等不同组织的MSCs在肿瘤进展中的作用以及肿瘤的异质性。通过收集MSCs的培养上清液作为MSCs条件培养基（CMs），本研究发现MSCs-CM对MDA-MB-231和A549肿瘤细胞的增殖没有显著影响。与MSCs-CM孵育后，MDA-MB-231细胞的迁移增强，而A549细胞的迁移受到抑制。此外，在经MSCs-CM处理的MDA-MB-231细胞中，胰岛素受体（IRs）的磷酸化上调，而在经MSCs-CM处理的A549细胞中，人表皮生长因子受体3（Her3）的磷酸化下调。综上所述，这些发现表明IR和Her3的磷酸化可能导致MSCs-CM对这两种细胞系迁移产生不同影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/b3cfc35e4b54/ol-13-03-1581-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/ed74ea73cc31/ol-13-03-1581-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/2f09c411d3c3/ol-13-03-1581-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/5cf6abc1cea1/ol-13-03-1581-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/b3cfc35e4b54/ol-13-03-1581-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/ed74ea73cc31/ol-13-03-1581-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/2f09c411d3c3/ol-13-03-1581-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/5cf6abc1cea1/ol-13-03-1581-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4de/5403177/b3cfc35e4b54/ol-13-03-1581-g03.jpg

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