Li Yueying, Liu Jun, Liu Yali, Yang Xiaoming, Huang Bingzhong, Chen Min
School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
School of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
Oncol Lett. 2017 Mar;13(3):1873-1879. doi: 10.3892/ol.2017.5664. Epub 2017 Feb 1.
Ginkgol C17:1 is a bioactive compound derived from . In the present study, the effect and possible mechanisms of action of Ginkgol C17:1 on the biological behaviors of tumor cells were investigated. Whilst cell proliferation was assessed using the MTT assay, the behaviors of cell migration and invasion were explored using Transwell and modified Transwell assays. The results revealed that Ginkgol C17:1 significantly inhibited the proliferation, migration and invasion of human tumor cells in a dose-dependent manner. Furthermore, due to their associations with the biological behaviors of tumor cells, the protein expression of matrix metalloproteinase (MMP)-7, Ras homolog gene family, member A (RhoA) and phosphorylated-protein kinase B (Akt) was analyzed by western blotting. The results showed that the expression of the aforementioned proteins was decreased markedly following Ginkgol C17:1 treatment. The results of the present study suggested that Ginkgol C17:1 suppresses the biological behaviors of tumor cells by inhibiting the activation of the mitogen-activated protein kinase/MMP, Rho/Rho-associated protein kinase and phosphatidylinositol 3-kinase/Akt signaling pathways.
银杏酚酸C17:1是一种源自……的生物活性化合物。在本研究中，研究了银杏酚酸C17:1对肿瘤细胞生物学行为的影响及其可能的作用机制。使用MTT法评估细胞增殖，使用Transwell和改良Transwell法探究细胞迁移和侵袭行为。结果显示，银杏酚酸C17:1以剂量依赖的方式显著抑制人肿瘤细胞的增殖、迁移和侵袭。此外，由于它们与肿瘤细胞生物学行为相关，通过蛋白质印迹法分析了基质金属蛋白酶（MMP）-7、Ras同源基因家族成员A（RhoA）和磷酸化蛋白激酶B（Akt）的蛋白表达。结果表明，银杏酚酸C17:1处理后上述蛋白的表达明显降低。本研究结果表明，银杏酚酸C17:1通过抑制丝裂原活化蛋白激酶/MMP、Rho/Rho相关蛋白激酶和磷脂酰肌醇3-激酶/Akt信号通路的激活来抑制肿瘤细胞的生物学行为。
