Faria Clarissa Perez, Zanini Graziela Maria, Dias Gisele Silva, Sousa Maria do Céu
CNC - Center for Neurosciences and Cell Biology, University of Coimbra, 3030-548 Coimbra, Portugal; Faculty of Pharmacy, University of Coimbra, 3030-548 Coimbra, Portugal; Laboratory of Parasitology, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Rio de Janeiro, 21040-900, Brazil.
Laboratory of Parasitology, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Rio de Janeiro, 21040-900, Brazil.
Acta Trop. 2017 Aug;172:80-85. doi: 10.1016/j.actatropica.2017.04.026. Epub 2017 Apr 27.
Giardia lamblia is an intestinal parasite that has an extensive genetic variation among isolates. This species is divided into eight different assemblages (A-H), but only assemblages A and B have been associated with human infections. Studies on the associations of G. lamblia assemblages and symptoms have been done but were inconclusive. The aim of this study was to correlate G. lamblia assemblages with symptoms in patients with and without HIV/AIDS and its association with the CD4T cell count. The cross-sectional survey was conducted among patients attending the Evandro Chagas National Institute of Infectious Diseases (INI/FIOCRUZ) in Rio de Janeiro from January 2011 to February 2015. Thirty-eight of 65 microscopically positive stool samples for G. lamblia were from HIV positive patients and 27 were from HIV negative patients. Of the HIV infected patients, 19 (55.9%) were genotyped as assemblage B of which 9 (47.4%) had a CD4Tcell count below 200cells/mm. In addition, we found a greater number of samples belonging to assemblage B in symptomatic cases (11 of 19; 57.9%). Our data suggest that assemblage B is very likely to be found in HIV infected patients and probably the lower CD4T count gives advantages for assemblage B replication. Furthermore, assemblage B seems to be associated with symptomatology, particularly abdominal pain, asthenia, diarrhea, fever, headache and myalgia. This study provides information on G. lamblia assemblages and symptoms in patients with and without HIV/AIDS virus and their association with CD4Tcell counts.
蓝氏贾第鞭毛虫是一种肠道寄生虫，不同分离株之间存在广泛的遗传变异。该物种分为八个不同的组合（A - H），但只有A和B组合与人类感染有关。关于蓝氏贾第鞭毛虫组合与症状之间关联的研究已经开展，但尚无定论。本研究的目的是将蓝氏贾第鞭毛虫组合与有无艾滋病毒/艾滋病患者的症状及其与CD4T细胞计数的关联进行对比。2011年1月至2015年2月，在里约热内卢的埃万德罗·查加斯国家传染病研究所（INI/FIOCRUZ）就诊的患者中进行了横断面调查。65份蓝氏贾第鞭毛虫粪便显微镜检查呈阳性的样本中，38份来自艾滋病毒阳性患者，27份来自艾滋病毒阴性患者。在艾滋病毒感染患者中，19例（55.9%）的基因型为B组合，其中9例（47.4%）的CD4T细胞计数低于200个细胞/mm³。此外，我们发现在有症状的病例中，属于B组合的样本数量更多（19例中的11例；57.9%）。我们的数据表明，B组合很可能在艾滋病毒感染患者中出现，而且CD4T细胞计数较低可能有利于B组合的复制。此外，B组合似乎与症状有关，特别是腹痛、乏力、腹泻、发热、头痛和肌痛。本研究提供了关于有无艾滋病毒/艾滋病病毒患者的蓝氏贾第鞭毛虫组合与症状及其与CD4T细胞计数关联的信息。
