安哥拉本戈综合医院5岁以下腹泻儿童蓝氏贾第鞭毛虫的分子特征分析
Molecular characterization of Giardia lamblia in children less than 5 years of age with diarrhoea attending the Bengo General Hospital, Angola.
作者信息
Gasparinho Carolina, Ferreira Filipa S, Mayer António Carlos, Mirante Maria Clara, Vaz Nery Susana, Santos-Reis Ana, Portugal-Calisto Daniela, Brito Miguel
机构信息
Clinical Research, Centro de Investigação em Saúde de Angola, Caxito, Angola.
Unidade de Saúde Pública Internacional e Bioestatística, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, 1349-008 Lisboa, Portugal.
出版信息
Trans R Soc Trop Med Hyg. 2017 Nov 1;111(11):497-503. doi: 10.1093/trstmh/try004.
BACKGROUND
Giardia lamblia is a pathogenic intestinal protozoan with high prevalence in developing countries, especially among children. Molecular characterization has revealed the existence of eight assemblages, with A and B being more commonly described in human infections. Despite its importance, to our knowledge this is the first published molecular analysis of G. lamblia assemblages in Angola.
METHODS
The present study aimed to identify the assemblages of G. lamblia in children with acute diarrhoea presenting at the Bengo General Hospital, Angola. A stool sample was collected and microscopy and immunochromatographic tests were used. DNA was extracted and assemblage determination was performed through amplification of the gene fragment ssu-rRNA (175 bp) and β-giardin (511 bp) through polymerase chain reaction and DNA sequencing.
RESULTS
Of the 16 stool samples screened, 12 were successfully sequenced. Eleven isolates were assigned to assemblage B and one to assemblage A. Subassemblage determination was not possible for assemblage B, while the single isolate assigned to assemblage A was identified as belonging to subassemblage A3.
CONCLUSION
This study provides information about G. lamblia assemblages in Bengo Province, Angola and may contribute as a first step in understanding the molecular epidemiology of this protozoan in the country. GenBank accession numbers for the ssur-RNA gene: MF479750, MF479751, MF479752, MF479753, MF479754, MF479755, MF479756, MF479757, MF479758, MF479759, MF479760, MF479761. GenBank accession numbers for the β-giardin gene: MF565378, MF565379, MF565380, MF565381.
背景
蓝氏贾第鞭毛虫是一种致病性肠道原生动物，在发展中国家尤其是儿童中普遍存在。分子特征分析显示存在八个组合，其中A和B在人类感染中更为常见。尽管其很重要，但据我们所知，这是安哥拉首次发表的关于蓝氏贾第鞭毛虫组合的分子分析。
方法
本研究旨在鉴定安哥拉本戈综合医院出现急性腹泻的儿童中蓝氏贾第鞭毛虫的组合。收集粪便样本并进行显微镜检查和免疫色谱试验。提取DNA，并通过聚合酶链反应和DNA测序扩增基因片段小亚基核糖体RNA（175 bp）和β-贾第虫蛋白（511 bp）来进行组合测定。
结果
在筛选的16份粪便样本中，12份成功测序。11个分离株被归为B组合，1个归为A组合。B组合无法进行亚组合测定，而归为A组合的单个分离株被鉴定为属于A3亚组合。
结论
本研究提供了安哥拉本戈省蓝氏贾第鞭毛虫组合的信息，可能作为了解该国这种原生动物分子流行病学的第一步。小亚基核糖体RNA基因的GenBank登录号：MF479750、MF479751、MF479752、MF479753、MF479754、MF479755、MF479756、MF479757、MF479758、MF479759、MF479760、MF479761。β-贾第虫蛋白基因的GenBank登录号：MF565378、MF565379、MF565380、MF565381。
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