用于活细胞和组织溶酶体中HO成像的双光子荧光探针的制备。

Preparation of a Two-Photon Fluorescent Probe for Imaging HO in Lysosomes in Living Cells and Tissues.

作者信息

Ren Mingguang, Deng Beibei, Kong Xiuqi, Tang Yonghe, Lin Weiying

机构信息

Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, YiFu Building, No 336 West Road of Nan Xinzhuang, Jinan, Shandong, 250022, P. R. China.

出版信息

Methods Mol Biol. 2017;1594:129-139. doi: 10.1007/978-1-4939-6934-0_7.

DOI:10.1007/978-1-4939-6934-0_7

PMID:28456979

Abstract

Hydrogen peroxide (HO) plays important roles in many physiological and pathological processes. At the cellular organelle level, the abnormal concentrations of HO in the lysosomes may cause redox imbalance and the loss of the critical functions of the lysosomes. Herein, we describe the preparation of a potent lysosome-targeted two-photon fluorescent probe (Lyso-HP) for the detection of HO in the lysosomes in the living cells. This unique fluorescent probe can also be employed to effectively detect HO in the living tissues using two-photon fluorescence microscopy.

摘要

过氧化氢（HO）在许多生理和病理过程中发挥着重要作用。在细胞器水平上，溶酶体中HO浓度异常可能导致氧化还原失衡以及溶酶体关键功能丧失。在此，我们描述了一种用于检测活细胞溶酶体中HO的高效溶酶体靶向双光子荧光探针（Lyso-HP）的制备方法。这种独特的荧光探针还可用于利用双光子荧光显微镜有效检测活组织中的HO。