Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, 06800, Turkey.
Life Sciences Application and Research Center, Gazi University, Ankara, 06830, Turkey; Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Ankara, 06500, Turkey.
Biomaterials. 2017 Jul;134:117-127. doi: 10.1016/j.biomaterials.2017.04.040. Epub 2017 Apr 22.
Burn injuries are one of the most common types of trauma worldwide, and their unique physiology requires the development of specialized therapeutic materials for their treatment. Here, we report the use of synthetic, functional and biodegradable peptide nanofiber gels for the improved healing of burn wounds to alleviate the progressive loss of tissue function at the post-burn wound site. These bioactive nanofiber gels form scaffolds that recapitulate the structure and function of the native extracellular matrix through signaling peptide epitopes, which can trigger angiogenesis through their affinity to basic growth factors. In this study, the angiogenesis-promoting properties of the bioactive scaffolds were utilized for the treatment of a thermal burn model. Following the excision of necrotic tissue, bioactive gels and control solutions were applied topically onto the wound area. The wound healing process was evaluated at 7, 14 and 21 days following injury through histological observations, immunostaining and marker RNA/protein analysis. Bioactive peptide nanofiber-treated burn wounds formed well-organized and collagen-rich granulation tissue layers, produced a greater density of newly formed blood vessels, and exhibited increased re-epithelialization and skin appendage development with minimal crust formation, while non-bioactive peptide nanofibers and the commercial wound dressing 3M™ Tegaderm™ did not exhibit significant efficiency over sucrose controls. Overall, the heparin-mimetic peptide nanofiber gels increased the rate of repair of burn injuries and can be used as an effective means of facilitating wound healing.
烧伤是全球最常见的创伤类型之一，其独特的生理学特性要求开发专门的治疗材料来治疗烧伤。在这里，我们报告了使用合成的、功能的和可生物降解的肽纳米纤维凝胶来改善烧伤伤口的愈合，以减轻烧伤伤口部位组织功能的渐进性丧失。这些生物活性纳米纤维凝胶形成支架，通过信号肽表位模拟天然细胞外基质的结构和功能，这些表位可以通过与基本生长因子的亲和力触发血管生成。在这项研究中，生物活性支架的促血管生成特性被用于治疗热烧伤模型。在切除坏死组织后，将生物活性凝胶和对照溶液局部应用于伤口区域。通过组织学观察、免疫染色和标记 RNA/蛋白质分析，在损伤后 7、14 和 21 天评估伤口愈合过程。用生物活性肽纳米纤维处理的烧伤伤口形成了组织有序、富含胶原蛋白的肉芽组织层，产生了更高密度的新形成的血管，并表现出更高的再上皮化和皮肤附属物发育，形成的痂最小，而非生物活性肽纳米纤维和商业伤口敷料 3M™ Tegaderm™ 与蔗糖对照相比没有表现出显著的效率。总的来说，肝素模拟肽纳米纤维凝胶加速了烧伤伤口的修复，可以作为促进伤口愈合的有效手段。
