Kandikattu Hemanth Kumar, Rachitha P, Jayashree G V, Krupashree K, Sukhith M, Majid Abdul, Amruta Narayanappa, Khanum Farhath
Biochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysore, India.
Seed Science and Technology Dept. Gandhi Krishi Vignana Kendra, Bengaluru, India.
Biomed Pharmacother. 2017 Jul;91:191-201. doi: 10.1016/j.biopha.2017.04.049. Epub 2017 Apr 28.
Elleteria repens is a large cardamom used in the culinary preparations. In the present study, we have evaluated in vitro antioxidant and free radical scavenging activities E. repens hexane extract (ERH) exhibited DPPH and metal chelating activity with IC values of 464±28.3μg/ml, 199±7.2μg/ml whereas the reducing power and antioxidant activities are found to be 289±14.6 AAE/mg, 468±22.7 GAE/mg. The observed antioxidant activities could be correlated with metabolites such as polyphenol, flavonoid, and terpenoid group of compounds identified in hexane fraction of E. repens by 4D GCXGC TOF-MS. Further ERH was evaluated for its protective properties against macromolecules such as DNA, protein and lipid damage. The extract showed protection against HO induced DNA damage and inhibited AAPH induced protein oxidation and lipid peroxidation. Moreover, ERH administration to rats at 50 and 100mg/kg inhibited carrageenan-induced paw edema, and down-regulated cytokines such as COX-2, IL-6, and TNF-α and inhibited i-NOS mediated NO generation. E. repens also exhibited antioxidant effects by restoring SOD, catalase, GSH levels and inhibited lipid peroxidation in carrageenan challenged rats. Overall, the results suggest that E. repens may be useful in combating inflammation and oxidative stress.
匍匐豆蔻是一种用于烹饪的大型豆蔻。在本研究中，我们评估了匍匐豆蔻己烷提取物（ERH）的体外抗氧化和自由基清除活性。ERH表现出DPPH和金属螯合活性，IC值分别为464±28.3μg/ml、199±7.2μg/ml，而还原能力和抗氧化活性分别为289±14.6 AAE/mg、468±22.7 GAE/mg。观察到的抗氧化活性可能与通过4D GCXGC TOF-MS在匍匐豆蔻己烷馏分中鉴定出的多酚、黄酮类和萜类化合物等代谢物相关。进一步评估了ERH对DNA、蛋白质和脂质等大分子的保护特性。该提取物显示出对羟基自由基诱导的DNA损伤的保护作用，并抑制了2,2'-偶氮二异丁基脒二盐酸盐诱导的蛋白质氧化和脂质过氧化。此外，以50和100mg/kg的剂量给大鼠施用ERH可抑制角叉菜胶诱导的爪肿胀，并下调细胞因子如COX-2、IL-6和TNF-α，并抑制诱导型一氧化氮合酶介导的一氧化氮生成。匍匐豆蔻还通过恢复角叉菜胶攻击的大鼠体内超氧化物歧化酶、过氧化氢酶、谷胱甘肽水平并抑制脂质过氧化而表现出抗氧化作用。总体而言，结果表明匍匐豆蔻可能有助于对抗炎症和氧化应激。
