Chakraborty Manodeep, Bhattacharjee Ananya, Kamath Jagadish Vasudev
Department of Pharmacology, Shree Devi College of Pharmacy, Mangalore, Karnataka, India.
Indian J Pharmacol. 2017 Jan-Feb;49(1):65-70. doi: 10.4103/0253-7613.201015.
Curcumin is a well-established cardioprotective phytoconstituent, but the poor bioavailability associated with it is always a matter of therapeutic challenge. The present study was undertaken to increase the therapeutic efficacy of curcumin by combining with bio-enhancer like piperine against cyclophosphamide (CP)-induced cardiotoxicity in rats.
Rats ( = 8) were treated with curcumin (200 mg/kg, p.o.) alone and different dose combination of curcumin (100, 50, 25 mg/kg, p.o.) and piperine (20 mg/kg, p.o.) for 10 days. All the treated groups were subjected to CP (200 mg/kg, i.p.) toxicity on day 1. Twenty-four hours after the last treatment, the effects were evaluated by changes in electrocardiographic (ECG) parameters, serum biomarkers, lipid profile, tissue antioxidants, and histopathological examination. Serum and tissue homogenate parameters were measured by semi-autoanalyzer and spectrophotometer, respectively. Results obtained were assessed by one-way analysis of variance followed by Tukey-Karmer multiple comparison test.
Incorporation of piperine with the doses of 50 and 25 mg/kg with curcumin exhibited significant beneficial effect compared to curcumin alone-treated group. The best effective group was a combination of curcumin 50 mg/kg with piperine 20 mg/kg which showed extremely significant ( < 0.001) decrease and increase in ECG and serum biomarker level, respectively, and moderate significant ( < 0.01) decrease in lipid profile, antioxidant levels, and histopathological score, compared to curcumin alone-treated group.
From this study, it can be concluded that a novel dose combination of curcumin (50 mg/kg) with piperine (20 mg/kg) exhibited profound cardioprotection compared to curcumin (200 mg/kg) alone-treated group.
姜黄素是一种公认的具有心脏保护作用的植物成分，但其较差的生物利用度一直是治疗方面的挑战。本研究旨在通过与胡椒碱等生物增强剂联合使用来提高姜黄素对环磷酰胺（CP）诱导的大鼠心脏毒性的治疗效果。
将大鼠（n = 8）分别单独用姜黄素（200 mg/kg，口服）以及姜黄素（100、50、25 mg/kg，口服）与胡椒碱（20 mg/kg，口服）的不同剂量组合进行处理，持续10天。所有处理组在第1天接受CP（200 mg/kg，腹腔注射）毒性处理。在最后一次处理后24小时，通过心电图（ECG）参数变化、血清生物标志物、血脂谱、组织抗氧化剂以及组织病理学检查来评估效果。血清和组织匀浆参数分别通过半自动分析仪和分光光度计进行测量。所得结果通过单因素方差分析，随后进行Tukey-Karmer多重比较检验进行评估。
与单独使用姜黄素处理组相比，50 mg/kg和25 mg/kg剂量的姜黄素与胡椒碱联合使用表现出显著的有益效果。最佳有效组是50 mg/kg姜黄素与20 mg/kg胡椒碱的组合，与单独使用姜黄素处理组相比，该组合分别使心电图和血清生物标志物水平极显著降低（P < 0.001）和升高，血脂谱、抗氧化剂水平以及组织病理学评分中度显著降低（P < 0.01）。
从本研究可以得出结论，与单独使用姜黄素（200 mg/kg）处理组相比，姜黄素（50 mg/kg）与胡椒碱（20 mg/kg）的新型剂量组合表现出显著的心脏保护作用。
