Borah Mukundam, Das Swarnamoni
Department of Pharmacology, Gauhati Medical College and Hospital, Guwahati, Assam, India.
Department of Pharmacology, Assam Medical College and Hospital, Dibrugarh, Assam, India.
Indian J Pharmacol. 2017 Jan-Feb;49(1):71-76. doi: 10.4103/0253-7613.201030.
To evaluate the antidiabetic, antihyperlipidemic, and antioxidant activities of the ethanolic extracts of the flowers and inflorescence stalk of Colla. in streptozotocin (STZ)-induced Type 1 diabetic rats.
Diabetes was induced in male Wistar albino rats (150-200 g) by single intraperitoneal injection of STZ (60 mg/kg b.w. i.p.). Albino rats ( = 25) were divided into five groups, of which five animals each. Group A (normal control) and Group B (diabetic control) received normal saline (10 ml/kg/day p.o.), whereas Group C and Group D received 250 mg/kg/day p.o. of flower and inflorescence stalk ethanolic extracts, respectively, for 2 weeks. Group E (diabetic standard) received 6 U/kg/day s.c of Neutral Protamine Hagedorn insulin. Fasting blood sugar, serum insulin, catalase (CAT), malondialdehyde (MDA), and serum lipid profile were estimated at specific intervals of time. Effect of the extracts on intestinal glucose absorption was also evaluated to know the probable mechanism of action.
Diabetic control exhibited significant increase in blood glucose, serum cholesterol, triglycerides, low-density lipoprotein, serum MDA levels and decreased serum CAT, and high-density lipoprotein levels which were significantly reverted by flower and inflorescence stalk ethanolic extracts after 2 weeks. Serum insulin levels were in increased ( < 0.05), and intestinal glucose absorption decreased significantly ( < 0.01) in extract-treated groups.
Flower and inflorescence stalk of Colla. possess significant antidiabetic, antihyperlipidemic, and antioxidant activities in STZ-induced Type 1 diabetic rats.
评估锦葵花花和花序梗乙醇提取物对链脲佐菌素（STZ）诱导的1型糖尿病大鼠的抗糖尿病、抗高血脂和抗氧化活性。
通过单次腹腔注射STZ（60mg/kg体重，腹腔注射）诱导雄性Wistar白化大鼠（150 - 200g）患糖尿病。将白化大鼠（n = 25）分为五组，每组五只动物。A组（正常对照组）和B组（糖尿病对照组）给予生理盐水（10ml/kg/天，口服），而C组和D组分别给予250mg/kg/天的花花和花序梗乙醇提取物，口服，持续2周。E组（糖尿病标准组）给予6U/kg/天的中性鱼精蛋白锌胰岛素皮下注射。在特定时间间隔测定空腹血糖、血清胰岛素、过氧化氢酶（CAT）、丙二醛（MDA）和血清脂质谱。还评估了提取物对肠道葡萄糖吸收的影响，以了解可能的作用机制。
糖尿病对照组血糖、血清胆固醇、甘油三酯、低密度脂蛋白、血清MDA水平显著升高，血清CAT和高密度脂蛋白水平降低，2周后花花和花序梗乙醇提取物可显著逆转这些变化。提取物处理组血清胰岛素水平升高（P < 0.05），肠道葡萄糖吸收显著降低（P < 0.01）。
锦葵花花和花序梗对STZ诱导的1型糖尿病大鼠具有显著的抗糖尿病、抗高血脂和抗氧化活性作用。
