乳酸乳球菌亚种双乙酰乳酸乳球菌DRC3的pNP40的7.6千碱基EcoRI片段上乳链菌肽抗性决定簇和复制起点的克隆
Cloning of nisin resistance determinant and replication origin on 7.6-kilobase EcoRI fragment of pNP40 from Streptococcus lactis subsp. diacetylactis DRC3.
作者信息
Froseth B R, Herman R E, McKay L L
机构信息
Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108.
出版信息
Appl Environ Microbiol. 1988 Aug;54(8):2136-9. doi: 10.1128/aem.54.8.2136-2139.1988.
Abstract
The nisin resistance determinant and an origin of replication on pNP40, a plasmid of about 60 kilobases that is present in Streptococcus lactis subsp. diacetylactis DRC3, was cloned on a 7.6-kilobase EcoRI fragment. When self-ligated, this fragment existed as an independent replicon (pFM011) and contained a 2.6-kilobase EcoRI-XbaI fragment encoding nisin resistance.
摘要
乳链菌肽抗性决定因子以及存在于乳酸乳球菌亚种双乙酰乳酸亚种DRC3中的约60千碱基大小的质粒pNP40上的一个复制起点，被克隆到一个7.6千碱基的EcoRI片段上。当该片段自身连接时，它作为一个独立的复制子（pFM011）存在，并包含一个编码乳链菌肽抗性的2.6千碱基的EcoRI-XbaI片段。
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