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利用 conjugal plasmid pNP40 构建乳球菌乳亚种 425A 的噬菌体抗性衍生物。

Construction of a Bacteriophage-Resistant Derivative of Lactococcus lactis subsp. lactis 425A by Using the Conjugal Plasmid pNP40.

机构信息

The National Dairy Products Research Centre, Moorepark, Fermoy, County Cork, Irish Republic.

出版信息

Appl Environ Microbiol. 1991 Dec;57(12):3405-9. doi: 10.1128/aem.57.12.3405-3409.1991.

DOI:10.1128/aem.57.12.3405-3409.1991
PMID:16348595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC183987/
Abstract

Lactococcus lactis subsp. lactis 425A is an atypical strain which excretes a high concentration of alpha-acetolactate when grown in milk. The conjugative lactococcal plasmid pNP40, which encodes phage and nisin resistance, was introduced to strain 425A by conjugation, using resistance to phage and nisin as a selection. No phage-nisin resistance mutants were encountered. Transconjugants display complete resistance at both 21 and 39 degrees C to those phage previously identified as lytic for 425A. Transconjugants lose their resistance characteristics when spontaneously cured of pNP40. The commercially important property of 425A-production of high levels of alpha-acetolactic acid-is unaffected by the presence of pNP40.

摘要

乳球菌乳亚种 425A 是一种非典型菌株，当在牛奶中生长时，会分泌出高浓度的α-乙酰乳酸。具有噬菌体和乳链菌肽抗性的可接合乳球菌质粒 pNP40 通过接合被引入到 425A 菌株中，利用对噬菌体和乳链菌肽的抗性作为选择。没有遇到噬菌体-乳链菌肽抗性突变体。转导子在 21 和 39 摄氏度下对以前鉴定为 425A 裂解的那些噬菌体完全具有抗性。转导子在自发消除 pNP40 时失去其抗性特征。425A 产生高水平α-乙酰乳酸的商业上重要特性不受 pNP40 的存在影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c6/183987/00e4052f1fdc/aem00065-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c6/183987/182fb593a39b/aem00065-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c6/183987/00e4052f1fdc/aem00065-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c6/183987/182fb593a39b/aem00065-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36c6/183987/00e4052f1fdc/aem00065-0017-a.jpg

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