Tarabees Reda, Elsayed Mohamed S A, Shawish Reyad, Basiouni Shereen, Shehata Awad A
Faculty of Veterinary Medicine, El Sadat City University, Sadat City, Minoufiya, Egypt.
J Infect Dev Ctries. 2017 Apr 30;11(4):314-319. doi: 10.3855/jidc.8043.
Salmonella enterica serovars Enteritidis and Typhimurium represent the major serovars associated with human salmonellosis. Contamination of meat products with these serovars is considered the main source of infection.
In this study, 100 raw chicken meat samples were investigated for the presence of Salmonella spp., which were subsequently identified based on biochemical and serological tests as well as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) profile. Furthermore, the isolated serovars were examined using multiplex polymerase chain reaction (PCR) for the presence of virulence genes suspected to have a role in infection.
S. Enteritidis was isolated from two samples (2%), while S. Typhimurium was isolated from three samples (3%) of chicken meat. Of the 17 examined virulence genes using multiplex PCR, the sitC, sopB, sifA, lpfC, spaN, sipB, invA, spiA, and msgA genes were detected in S. Enteritidis. However, the sitC, iroN, sopB, sifA, lpfC, spaN, sipB, invA, and tolC genes were successfully amplified in S. Typhimurium.
The detection of S. Enteritidis and S. Typhimurium in meat, even at low incidence, has important implications. In addition, the data presented here is the first attempt to identify a wide range of virulence genes in Egyptian Salmonella isolates recovered from meat products. A strict public health and food safety regime is urgently needed in order to decrease the human health hazard risk associated with salmonellosis.
肠炎沙门氏菌血清型肠炎亚种和鼠伤寒亚种是与人类沙门氏菌病相关的主要血清型。这些血清型对肉类产品的污染被认为是主要感染源。
在本研究中，对100份生鸡肉样本进行了沙门氏菌属检测，随后通过生化和血清学检测以及基质辅助激光解吸/电离飞行时间（MALDI-TOF）图谱对其进行鉴定。此外，使用多重聚合酶链反应（PCR）对分离出的血清型检测是否存在怀疑在感染中起作用的毒力基因。
从两份样本（2%）中分离出肠炎沙门氏菌，从三份鸡肉样本（3%）中分离出鼠伤寒沙门氏菌。在使用多重PCR检测的17个毒力基因中，在肠炎沙门氏菌中检测到了sitC、sopB、sifA、lpfC、spaN、sipB、invA、spiA和msgA基因。然而，在鼠伤寒沙门氏菌中成功扩增出了sitC、iroN、sopB、sifA、lpfC、spaN、sipB、invA和tolC基因。
即使在肉类中肠炎沙门氏菌和鼠伤寒沙门氏菌的检出率较低，但这仍具有重要意义。此外，本文所提供的数据是首次尝试在从肉类产品中分离出的埃及沙门氏菌菌株中鉴定多种毒力基因。迫切需要建立严格的公共卫生和食品安全制度，以降低与沙门氏菌病相关的人类健康危害风险。
