In the present study, a total of 720 samples were collected from retail raw meat from 13 upazilas in Sylhet District, Bangladesh, of which 225 samples were from cattle meat, 210 samples were from goat meat, and 285 samples were from chicken meat. serovars Typhimurium and Enteritidis were screened for extended-spectrum β-lactamase (ESBL) genes using multiplex PCR. Among the 720 samples, spp. was detected in 28.06% (202 out of 720) of the samples, with Enteritidis and Typhimurium were identified in 11.53% (83 out of 720) and 12.22% (88 out of 720) of the samples, respectively. It was found that all serovars isolated from cattle meat displayed multidrug resistance (MDR) based on antimicrobial susceptibility testing. Notably, a significant proportion of Enteritidis isolates and all Typhimurium isolates from goat meat demonstrated complete resistance to multiple drugs (ampicillin, cefuroxime, and ceftazidime). Regarding chicken meat, out of 89 isolates encompassing both Typhimurium and Enteritidis, 57 isolates (64.04%) exhibited MDR. Additionally, exhibited the highest occurrence at 15.69% for Typhimurium and 7.89% for Enteritidis in chicken meat. Moreover, was only detected at 3.92% for Enteritidis in chicken meat. Furthermore, had the highest prevalence rate of 19.04% for Enteritidis and 25.80% for Typhimurium in cattle meat, followed by chicken meat. These findings highlight the urgency for monitoring ESBL-producing in retail raw meat and the need for strict measure to manage antibiotic use to prevent the spread of multidrug-resistant and ESBL-producing strains, thereby protecting humans and reducing public health risks.