Rahman Md Mahfujur, Hossain Hemayet, Chowdhury Md Shahidur Rahman, Hossain Md Mukter, Saleh Asmaa, Binsuwaidan Reem, Noreddin Ayman, Helmy Yosra A, El Zowalaty Mohamed E
Department of Medicine, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
Department of Anatomy and Histology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
Antibiotics (Basel). 2024 Jun 24;13(7):586. doi: 10.3390/antibiotics13070586.
In the present study, a total of 720 samples were collected from retail raw meat from 13 upazilas in Sylhet District, Bangladesh, of which 225 samples were from cattle meat, 210 samples were from goat meat, and 285 samples were from chicken meat. serovars Typhimurium and Enteritidis were screened for extended-spectrum β-lactamase (ESBL) genes using multiplex PCR. Among the 720 samples, spp. was detected in 28.06% (202 out of 720) of the samples, with Enteritidis and Typhimurium were identified in 11.53% (83 out of 720) and 12.22% (88 out of 720) of the samples, respectively. It was found that all serovars isolated from cattle meat displayed multidrug resistance (MDR) based on antimicrobial susceptibility testing. Notably, a significant proportion of Enteritidis isolates and all Typhimurium isolates from goat meat demonstrated complete resistance to multiple drugs (ampicillin, cefuroxime, and ceftazidime). Regarding chicken meat, out of 89 isolates encompassing both Typhimurium and Enteritidis, 57 isolates (64.04%) exhibited MDR. Additionally, exhibited the highest occurrence at 15.69% for Typhimurium and 7.89% for Enteritidis in chicken meat. Moreover, was only detected at 3.92% for Enteritidis in chicken meat. Furthermore, had the highest prevalence rate of 19.04% for Enteritidis and 25.80% for Typhimurium in cattle meat, followed by chicken meat. These findings highlight the urgency for monitoring ESBL-producing in retail raw meat and the need for strict measure to manage antibiotic use to prevent the spread of multidrug-resistant and ESBL-producing strains, thereby protecting humans and reducing public health risks.
在本研究中，从孟加拉国锡尔赫特地区13个乡的零售生肉中总共采集了720份样本，其中225份样本来自牛肉，210份样本来自山羊肉，285份样本来自鸡肉。使用多重PCR筛选鼠伤寒血清型和肠炎血清型中的超广谱β-内酰胺酶（ESBL）基因。在720份样本中，在28.06%（720份中的202份）的样本中检测到该菌属，肠炎血清型和鼠伤寒血清型分别在11.53%（720份中的83份）和12.22%（720份中的88份）的样本中被鉴定出来。基于抗菌药敏试验发现，从牛肉中分离出的所有该菌属血清型均表现出多重耐药性（MDR）。值得注意的是，从山羊肉中分离出的相当一部分肠炎血清型菌株和所有鼠伤寒血清型菌株对多种药物（氨苄西林、头孢呋辛和头孢他啶）表现出完全耐药。关于鸡肉，在89株包含鼠伤寒血清型和肠炎血清型的分离株中，57株（64.04%）表现出多重耐药性。此外，在鸡肉中，鼠伤寒血清型的出现率最高，为15.69%，肠炎血清型为7.89%。此外，肠炎血清型在鸡肉中的检出率仅为3.92%。此外，肠炎血清型在牛肉中的流行率最高，为19.04%，鼠伤寒血清型为25.80%，其次是鸡肉。这些发现凸显了监测零售生肉中产生ESBL的该菌属的紧迫性，以及采取严格措施管理抗生素使用以防止多重耐药和产生ESBL的该菌属菌株传播的必要性，从而保护人类并降低公共卫生风险。
