Barac Deng, Abdel-Mawgood Ahmed, Awad Sameh, Ghazy Mohamed, Mansour Hussein
Biotechnology Program, Basic and Applied Sciences, Egypt-Japan University of Science and Technology, Alexandria, Egypt.
Department of Microbiology, College of Veterinary Science, University of Bahr El Ghazal, Wau, South, Sudan.
Mol Biol Rep. 2024 Jul 13;51(1):794. doi: 10.1007/s11033-024-09704-1.
Salmonellosis is a widespread zoonotic disease that poses a significant threat to livestock and public health. This study aimed to serotype 20 Salmonella isolates obtained from sixty retail chicken meats, assess Salmonella contamination from eggs, and evaluate antibiotic resistance profiles.
Twenty eggs were randomly collected in the new Borg El Arab market. Bacterial isolation was carried out utilizing both traditional culture, biochemical, and PCR methods. Among the twenty eggs analyzed, three (15%) tested positive for Salmonella, while the remaining seventeen (85%) were confirmed as negative. Genotyping through multiplex PCR revealed the presence of two S. Enteritidis and other serovar, with the use of three specific gene sets: a random sequence for Salmonella spp., sdfI gene for S. Enteritidis, and flagellin (fliC gene) for S. Typhimurium. Out of the 20 isolates obtained from chicken meat, five (25%) were identified as S. Typhimurium, and three (15%) were classified as S. Enteritidis. All isolates sourced from chicken meat exhibited resistance to Rifampicin and Amoxicillin, with 90% displaying sensitivity to cefotaxime, gemifloxacin, and Erythromycin. Importantly, S. Blegdam, identified via serological methods, displayed resistance to all tested antibiotics. For the three isolates obtained from eggs, 66.6% showed sensitivity to cefotaxime, erythromycin, cefuraxime, and cefaclor, while displaying complete resistance (100%) to Amoxicillin, rifampicin, clarithromycin, and cefadroxil. Notably, one serovar exhibited absolute resistance to all tested drugs.
Stakeholders must implement strict control measures and rationalize antibiotic use in veterinary and human medicine due to the rise of antibiotic-resistant strains.
沙门氏菌病是一种广泛传播的人畜共患病，对家畜和公共卫生构成重大威胁。本研究旨在对从60份零售鸡肉中分离出的20株沙门氏菌进行血清型鉴定，评估鸡蛋中的沙门氏菌污染情况，并评估抗生素耐药性谱。
在新博格艾尔阿拉伯市场随机收集了20枚鸡蛋。采用传统培养、生化和PCR方法进行细菌分离。在分析的20枚鸡蛋中，3枚（15%）沙门氏菌检测呈阳性，其余17枚（85%）被确认为阴性。通过多重PCR进行基因分型，利用三组特定基因揭示了两种肠炎沙门氏菌和其他血清型的存在：沙门氏菌属的随机序列、肠炎沙门氏菌的sdfI基因以及鼠伤寒沙门氏菌的鞭毛蛋白（fliC基因）。从鸡肉中获得的20株分离株中，5株（25%）被鉴定为鼠伤寒沙门氏菌，3株（15%）被归类为肠炎沙门氏菌。所有源自鸡肉的分离株均对利福平阿莫西林耐药，90%对头孢噻肟、吉米沙星和红霉素敏感。重要的是，通过血清学方法鉴定的布莱格丹沙门氏菌对所有测试抗生素均耐药。对于从鸡蛋中获得的3株分离株，66.6%对头孢噻肟、红霉素、头孢呋辛和头孢克洛敏感，而对阿莫西林、利福平、克拉霉素和头孢羟氨苄完全耐药（100%）。值得注意的是，一种血清型对所有测试药物均表现出绝对耐药性。
由于抗生素耐药菌株的增加，利益相关者必须在兽医和人类医学中实施严格的控制措施并合理使用抗生素。
