Ramatla Tsepo, Khasapane Ntelekwane G, Mlangeni Lungile N, Mokgokong Prudent, Ramaili Taole, Ndou Rendani, Nkhebenyane Jane S, Lekota Kgaugelo, Thekisoe Oriel
Centre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South Africa.
Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2531, South Africa.
Antibiotics (Basel). 2024 May 16;13(5):458. doi: 10.3390/antibiotics13050458.
Rapid growth in commercial poultry production is one of the major sources of infections that leads to human salmonellosis. The two main serovars associated with human salmonellosis are enteritidis and typhimurium. The aim of this study was to determine the prevalence of serovars Enteritidis and Typhimurium as well as their pathogenicity islands (SPI) and antibiotic resistance profiles in broiler chicken feces from slaughterhouses. A total of 480 fecal samples from broiler chickens that were grouped into 96 pooled samples were identified to have spp. using the gene, whilst the and genes were used to screen for the presence of Enteritidis and Typhimurium serovars, respectively, by polymerase chain reaction (PCR) assays. The isolates were also screened for the presence of pathogenicity islands (SPIs) using PCR. The disc diffusion assay was performed to determine the antibiotic resistance profiles of the isolates. A total of 36 isolates were confirmed as spp. through amplification of the gene. Out of 36 confirmed spp. a total of 22 isolates were classified as . Enteritidis (n = 8) and were Typhimurium (n = 14) serovars. All (n = 22) Enteritidis and Typhimurium isolates possessed the (SPI-1), (SPI-2) and (SPI-11) pathogenicity islands genes. Amongst these serovars, 50% of the isolates (n = 11/22) were resistant to tetracycline and nalidixic acid. Only 22% of the isolates, Typhimurium (13.6%) and Enteritidis (9.1%) demonstrated resistance against three or more antibiotic classes. The most detected antibiotic resistance genes were (), , and with 13 (59.1%), 9 (40.9%), 9 (40.9%) and 7 (31.8%), respectively. The findings of this study revealed that Typhimurium is the most prevalent serotype detected in chicken feces. To reduce the risk to human health posed by salmonellosis, a stringent public health and food safety policy is required.
商业家禽生产的快速增长是导致人类沙门氏菌病感染的主要来源之一。与人类沙门氏菌病相关的两个主要血清型是肠炎沙门氏菌和鼠伤寒沙门氏菌。本研究的目的是确定屠宰场肉鸡粪便中肠炎沙门氏菌和鼠伤寒沙门氏菌血清型的流行情况，以及它们的致病岛（SPI）和抗生素耐药谱。使用基因对总共480份肉鸡粪便样本（分为96个混合样本）进行鉴定，确定含有 spp.，同时分别使用基因和基因，通过聚合酶链反应（PCR）检测筛选肠炎沙门氏菌和鼠伤寒沙门氏菌血清型的存在情况。还使用PCR对分离株进行致病岛（SPIs）的筛选。采用纸片扩散法测定分离株的抗生素耐药谱。通过基因扩增，共确认36株分离株为 spp.。在36株确诊的 spp.中，共有22株被分类为。肠炎沙门氏菌（n = 8）和鼠伤寒沙门氏菌（n = 14）血清型。所有（n = 22）肠炎沙门氏菌和鼠伤寒沙门氏菌分离株都具有（SPI-1）、（SPI-2）和（SPI-11）致病岛基因。在这些血清型中，50%的分离株（n = 11/22）对四环素和萘啶酸耐药。只有22%的分离株，即鼠伤寒沙门氏菌（13.6%）和肠炎沙门氏菌（9.1%）对三种或更多类别的抗生素表现出耐药性。检测到的最常见抗生素耐药基因分别是（）、、和，分别有13株（59.1%）、9株（40.9%）、9株（40.9%）和7株（31.8%）。本研究结果表明，鼠伤寒沙门氏菌是在鸡粪便中检测到的最常见血清型。为降低沙门氏菌病对人类健康造成的风险，需要严格的公共卫生和食品安全政策。
