胰岛素在体内对大鼠心脏磷酸果糖激酶-2的激活作用。

Activation of rat heart phosphofructokinase-2 by insulin in vivo.

作者信息

Rider M H, Hue L

出版信息

FEBS Lett. 1984 Oct 29;176(2):484-8. doi: 10.1016/0014-5793(84)81223-5.

Abstract

Fifteen minutes after the intravenous injection of overnight starved, anaesthetized rats with insulin, the concentration of fructose 2,6-biphosphate was increased more than 2-fold in the hearts of these animals. Insulin injection also caused a 2-3 fold increase in the Vmax of phosphofructokinase-2 with no detectable change in Km values. The effect persisted after precipitation of the enzyme with polyethylene glycol or after gel filtration through Sephadex G-25.

摘要

对禁食过夜、麻醉后的大鼠静脉注射胰岛素15分钟后，这些动物心脏中果糖2,6 -二磷酸的浓度增加了两倍多。注射胰岛素还使磷酸果糖激酶-2的Vmax增加了2 - 3倍，而Km值没有可检测到的变化。用聚乙二醇沉淀酶或通过Sephadex G - 25凝胶过滤后，这种效应仍然存在。