Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstrasse 1, Neuherberg D-85764, Germany.
FEMS Yeast Res. 2017 Jun 1;17(4). doi: 10.1093/femsyr/fox025.
Zygosaccharomyces bailii is one of the most problematic spoilage yeast species found in the food and beverage industry particularly in acidic products, due to its exceptional resistance to weak acid stress. This article describes the annotation of the genome sequence of Z. bailii IST302, a strain recently proven to be amenable to genetic manipulations and physiological studies. The work was based on the annotated genomes of strain ISA1307, an interspecies hybrid between Z. bailii and a closely related species, and the Z. bailii reference strain CLIB 213T. The resulting genome sequence of Z. bailii IST302 is distributed through 105 scaffolds, comprising a total of 5142 genes and a size of 10.8 Mb. Contrasting with CLIB 213T, strain IST302 does not form cell aggregates, allowing its manipulation in the laboratory for genetic and physiological studies. Comparative cell cycle analysis with the haploid and diploid Saccharomyces cerevisiae strains BY4741 and BY4743, respectively, suggests that Z. bailii IST302 is haploid. This is an additional trait that makes this strain attractive for the functional analysis of non-essential genes envisaging the elucidation of mechanisms underlying its high tolerance to weak acid food preservatives, or the investigation and exploitation of the potential of this resilient yeast species as cell factory.
毕赤酵母是食品和饮料工业中最具问题的污染酵母物种之一，特别是在酸性产品中，因为它对弱酸胁迫具有非凡的抵抗力。本文描述了 Z. bailii IST302 基因组序列的注释，该菌株最近被证明易于遗传操作和生理研究。这项工作基于 Z. bailii 和密切相关物种的种间杂种 ISA1307 菌株以及 Z. bailii 参考菌株 CLIB 213T 的注释基因组。Z. bailii IST302 的基因组序列分布在 105 个支架中，总共包含 5142 个基因，大小为 10.8 Mb。与 CLIB 213T 不同，菌株 IST302 不会形成细胞聚集体，允许在实验室中对其进行遗传和生理研究的操作。与分别为单倍体和二倍体的酿酒酵母菌株 BY4741 和 BY4743 的细胞周期比较分析表明，Z. bailii IST302 是单倍体。这是一个额外的特征，使该菌株成为非必需基因功能分析的有吸引力的选择，设想阐明其对弱酸食品防腐剂的高耐受性的机制，或调查和利用这种有弹性的酵母物种作为细胞工厂的潜力。
