Khan Junad, Noboru Noma, Young Andrew, Thomas Davis
Orofacial Pain Clinic, Eastman Institute for Oral Health, 625 Elmwood Ave, Rochester, NY,14620, USA.
Department of Oral Diagnostic Sciences, Nihon University School of Dentistry, Tokyo, Japan.
Pathophysiology. 2017 Sep;24(3):155-159. doi: 10.1016/j.pathophys.2017.04.001. Epub 2017 Apr 11.
Traumatic neuroma is neuronal tissue proliferation developed in a nerve injury site, often associated with increased sensitivity and spontaneous or evoked neuropathic pain. The mechanisms leading to the disorganized nerve proliferation are not completely understood, though inflammation in the injured nerve vicinity most likely has a role in the process. Inflammatory cytokines are also known to be involved in the maintenance and development of post-traumatic and neuropathic pain. The goal of this study was to quantify and compare pro and anti-inflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-10) levels in nerves that formed neuromas and nerves that did not, following sciatic nerve transection. A total of 30 rats were used in this study. Twenty rats underwent sciatic nerve transection and 10 underwent sham surgery. Six weeks post-surgery nerve sections were collected and histologically evaluated for neuroma formation. The samples were then classified as neuroma, non-neuroma and sham groups. TNF-α, IL-1β, IL-6 and IL-10 levels were measured in the nerves employing ELISA. TNF-α levels were significantly higher in both neuroma and non-neuroma-forming injured nerves compared to the sham group. IL-1β and IL-6 levels were significantly higher in the neuroma-forming nerves compared to the sham group. IL-10 levels were significantly higher in the non-neuroma group compared to the sham group. In conclusion IL-6, and IL-1 β may have a role in the formation of traumatic neuroma while IL-10 may inhibit neuroma formation.
创伤性神经瘤是在神经损伤部位形成的神经元组织增生，常伴有敏感性增加以及自发性或诱发性神经性疼痛。尽管受伤神经附近的炎症很可能在这一过程中发挥作用，但导致神经无序增生的机制尚未完全明确。已知炎性细胞因子也参与创伤后和神经性疼痛的维持与发展。本研究的目的是量化并比较坐骨神经横断后形成神经瘤的神经和未形成神经瘤的神经中促炎和抗炎细胞因子（肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6和白细胞介素-10）的水平。本研究共使用了30只大鼠。20只大鼠接受了坐骨神经横断手术，10只接受了假手术。术后六周收集神经切片并进行组织学评估以确定神经瘤形成情况。然后将样本分为神经瘤组、非神经瘤组和假手术组。采用酶联免疫吸附测定法测量神经中肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6和白细胞介素-10的水平。与假手术组相比，神经瘤组和形成非神经瘤的损伤神经中的肿瘤坏死因子-α水平均显著更高。与假手术组相比，形成神经瘤的神经中的白细胞介素-1β和白细胞介素-6水平显著更高。与假手术组相比，非神经瘤组中的白细胞介素-10水平显著更高。总之，白细胞介素-6和白细胞介素-1β可能在创伤性神经瘤的形成中起作用，而白细胞介素-10可能抑制神经瘤形成。
