Curran J, Kolakofsky D
Department of Microbiology, University of Geneva Medical School, Switzerland.
EMBO J. 1988 Sep;7(9):2869-74. doi: 10.1002/j.1460-2075.1988.tb03143.x.
Both peptide antisera and monoclonal antibodies detect a 10-kd protein (X) in Sendai virus infected cells, which represents approximately the last 95 residues of the 568-amino-acid-long P protein. The X protein does not appear to be derived from a precursor P in vivo, and in in vitro X can be made under conditions in which P synthesis has been arrested by hybridized DNA fragments or specific cleavage of the mRNA. X initiation is nevertheless cap dependent. Ribosomes which initiate X appear to pass directly from the cap to the initiation codon.
肽抗血清和单克隆抗体均可在仙台病毒感染的细胞中检测到一种10-kd的蛋白质（X），它大约代表568个氨基酸长的P蛋白的最后95个残基。X蛋白在体内似乎并非源自前体P，并且在体外，可在通过杂交DNA片段或mRNA的特异性切割使P合成停止的条件下产生X。然而，X的起始依赖于帽子结构。起始合成X的核糖体似乎直接从帽子结构转移到起始密码子。
