Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Department of Obstetrics and Gynecology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan.
J Formos Med Assoc. 2018 Mar;117(3):204-211. doi: 10.1016/j.jfma.2017.03.011. Epub 2017 Apr 29.
BACKGROUND/PURPOSE: To investigate the M1/M2 polarity of macrophages in the endometrium among different menstrual cycles, normal and abnormal pregnancies, and unexplained recurrent spontaneous abortions (RSAs).
Endometrial tissue was obtained from 43 patients undergoing hysterectomy, either in the follicular phase (Group 1, n = 23) or in the luteal phase (Group 2, n = 20). In addition, decidual tissue was obtained from 53 pregnant women during the first trimester, either of normal pregnancies (Group 3, n = 12) or abnormal pregnancies (Group 4: spontaneous abortions, n = 20; Group 5: unexplained RSA, n = 21). Using immunofluorescence to examine the M1 and M2 macrophages in the endometrium and deciduae from cases with different menstrual phases and various pregnancy outcomes, respectively, we endeavored to learn the possible pathophysiology of abortions.
M1 macrophages were abundant in the deciduae of spontaneous abortions and unexplained RSA, whereas the frequency of M2 macrophages was significantly higher in the endometrium of luteal phase and normal pregnancies.
M2 polarization is important for early successful pregnancies in humans.
背景/目的：研究不同月经周期、正常和异常妊娠以及不明原因复发性自然流产（RSA）中子宫内膜中巨噬细胞的 M1/M2 极性。
从 43 例行子宫切除术的患者的子宫内膜组织中获得，这些患者分别处于卵泡期（第 1 组，n=23）或黄体期（第 2 组，n=20）。此外，从 53 名孕早期的孕妇的蜕膜组织中获得，这些孕妇分别为正常妊娠（第 3 组，n=12）或异常妊娠（第 4 组：自然流产，n=20；第 5 组：不明原因 RSA，n=21）。通过免疫荧光检测不同月经周期和不同妊娠结局的子宫内膜和蜕膜中的 M1 和 M2 巨噬细胞，我们试图了解流产的可能病理生理学。
M1 巨噬细胞在自然流产和不明原因 RSA 的蜕膜中丰富，而 M2 巨噬细胞在黄体期和正常妊娠的子宫内膜中的频率明显更高。
M2 极化对人类早期成功妊娠很重要。
