Song Andrew T, Galli Antonella, Leclerc Severine, Nattel Stanley, Mandato Craig, Andelfinger Gregor
Cardiovascular Genetics, Department of Pediatrics, CHU Sainte-Justine, Montreal, QC, Canada; McGill University, Department of Anatomy and Cell Biology, Montreal, QC, Canada.
Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Gene Expr Patterns. 2017 Nov;25-26:36-45. doi: 10.1016/j.gep.2017.04.004. Epub 2017 Apr 29.
SGO1 has been characterized in its function in correct cell division and its role in centrosome cohesion in the nucleus. However, its organ-specific maturation-related expression pattern in vivo remains largely uncharacterized. Here, we show clear SGO1 expression in post-developmental neuronal cells and cytoplasmic localisation in nucleated cells with a transgenic mice model and immunohistochemistry of wild type mice. We demonstrate extranuclear expression of Sgo1 in the developing heart and gut, which have been shown to be dysregulated in humans with homozygous SGO1 mutation. Additionally, we show Sgo1 expression in select population of retinal cells in developing and post-developmental retina. Our expression analysis strongly suggests that the function of SGO1 goes beyond its well characterized role in cell division.
SGO1在正确的细胞分裂中的功能及其在细胞核中心体黏附中的作用已得到明确。然而，其在体内器官特异性成熟相关的表达模式在很大程度上仍未得到充分研究。在这里，我们通过转基因小鼠模型和野生型小鼠的免疫组织化学方法，在发育后的神经元细胞中显示出清晰的SGO1表达，并在有核细胞中观察到其细胞质定位。我们证明了Sgo1在发育中的心脏和肠道中的核外表达，在患有纯合SGO1突变的人类中，这些组织已被证明存在失调。此外，我们还显示了Sgo1在发育中和发育后的视网膜中特定视网膜细胞群体中的表达。我们的表达分析强烈表明，SGO1的功能超出了其在细胞分裂中已明确的作用。
