Baig Abdul M, Lalani Salima, Khan Naveed A
Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.
Faculty of Science and Technology, Department of Biological Sciences, Sunway University, Bandar Sunway, Selangor, Malaysia.
J Basic Microbiol. 2017 Jul;57(7):574-579. doi: 10.1002/jobm.201700025. Epub 2017 May 3.
Here we describe features of apoptosis in unicellular Acanthamoeba castellanii belonging to the T4 genotype. When exposed to apoptosis-inducing compounds such as doxorubicin, A. castellanii trophozoites exhibited cell shrinkage and membrane blebbing as observed microscopically, DNA fragmentation using agarose gel electrophoresis, and phosphatidylserine (PS) externalization using annexin V immunostaining. Overall, these findings suggest the existence of apoptosis in A. castellanii possibly mediated by intrinsic apoptotic cascade. Further research in this field could provide avenues to selectively induce apoptosis in A. castellanii by triggering intrinsic apoptotic cascade.
在此，我们描述了属于T4基因型的单细胞卡氏棘阿米巴细胞凋亡的特征。当暴露于阿霉素等凋亡诱导化合物时，卡氏棘阿米巴滋养体在显微镜下呈现细胞收缩和膜泡形成，通过琼脂糖凝胶电泳检测到DNA片段化，并通过膜联蛋白V免疫染色检测到磷脂酰丝氨酸（PS）外化。总体而言，这些发现表明卡氏棘阿米巴存在可能由内源性凋亡级联介导的细胞凋亡。该领域的进一步研究可为通过触发内源性凋亡级联来选择性诱导卡氏棘阿米巴细胞凋亡提供途径。
