Gompels U A, Craxton M A, Honess R W
Division of Virology, National Institute for Medical Research, Mill Hill, London, U.K.
J Gen Virol. 1988 Nov;69 ( Pt 11):2819-29. doi: 10.1099/0022-1317-69-11-2819.
We present the nucleotide sequence of the glycoprotein H (gH) gene of herpesvirus saimiri (HVS), a representative of the T lymphotropic herpesviruses of New World monkeys, and compare the predicted amino acid sequence with sequences of homologous proteins from four human herpesviruses. The HVS gH gene is located within a block of genes encoding products conserved in all herpesvirus subgroups as represented by the human herpesviruses herpes simplex virus, varicella-zoster virus, cytomegalovirus and Epstein-Barr virus. In agreement with the biological grouping of HVS as a lymphotropic gammaherpesvirus, its gH amino acid sequence shows greatest similarity to that of the B lymphotropic Epstein-Barr virus, although the nucleotide sequences of their respective gH genes show little similarity given different G + C compositions of 31% and 54%. The similarity observed between the gH amino acid sequences of the two representative gammaherpesviruses is greater than that between the two human alphaherpesviruses varicella-zoster virus and herpes simplex virus. The members of the gH family range in size from 706 to 743 amino acid residues for the beta- and gammaherpesviruses, to 838 to 841 for the alphaherpesviruses, giving non-glycosylated precursors with Mr values of 78,322 to 93,651. The difference in size is due to heterogeneity in the poorly conserved N-terminal regions of the larger alphaherpesviruses compared to the smaller beta- and gammaherpesvirus molecules. Greatest similarity is observed in the C-terminal halves of the proteins including residues surrounding four conserved cysteine residues, a conserved N-linked glycosylation site (within the sequence NGTV) 13 to 18 residues proximal to the membrane-spanning sequences, and a short cytoplasmic domain of seven or eight residues for the beta- and gammaherpesviruses' and 14 or 15 residues for the alphaherpesviruses' gH. Thus, the representatives of all subgroups of herpesviruses, including those with a non-human host, encode gH homologues. Together with the observation that gH of these viruses are major targets for virus neutralization by antibody, this suggests that this glycoprotein family is essential among all herpesviruses and represents a major component involved in herpesvirus infectivity.
我们展示了松鼠猴疱疹病毒（HVS）糖蛋白H（gH）基因的核苷酸序列，HVS是新大陆猴嗜T淋巴细胞疱疹病毒的代表，并将预测的氨基酸序列与四种人类疱疹病毒同源蛋白的序列进行了比较。HVS gH基因位于一组基因中，这些基因编码的产物在所有疱疹病毒亚组中都是保守的，以人类疱疹病毒单纯疱疹病毒、水痘-带状疱疹病毒、巨细胞病毒和爱泼斯坦-巴尔病毒为代表。与HVS作为嗜淋巴细胞γ疱疹病毒的生物学分类一致，其gH氨基酸序列与嗜B淋巴细胞的爱泼斯坦-巴尔病毒的序列相似度最高，尽管它们各自gH基因的核苷酸序列由于31%和54%的不同G + C组成而相似度很低。两种代表性γ疱疹病毒的gH氨基酸序列之间的相似性大于两种人类α疱疹病毒水痘-带状疱疹病毒和单纯疱疹病毒之间的相似性。gH家族成员的大小范围从β和γ疱疹病毒的706至743个氨基酸残基，到α疱疹病毒的838至841个氨基酸残基，产生的非糖基化前体的Mr值为78,322至93,651。大小差异是由于与较小的β和γ疱疹病毒分子相比，较大α疱疹病毒保守性较差的N端区域存在异质性。在蛋白质的C端半部观察到最大的相似性，包括围绕四个保守半胱氨酸残基的残基、一个保守的N-连接糖基化位点（在序列NGTV内），位于跨膜序列近端13至18个残基处，以及β和γ疱疹病毒的gH有七个或八个残基的短细胞质结构域，α疱疹病毒的gH有14或15个残基。因此，疱疹病毒所有亚组的代表，包括那些以非人类为宿主的，都编码gH同源物。再加上观察到这些病毒的gH是抗体中和病毒的主要靶点，这表明这个糖蛋白家族在所有疱疹病毒中都是必不可少的，并且是参与疱疹病毒感染性的主要成分。
