Bublot M, Lomonte P, Lequarre A S, Albrecht J C, Nicholas J, Fleckenstein B, Pastoret P P, Thiry E
Virology Department, Faculty of Veterinary Medicine, University of Liège, Belgium.
Virology. 1992 Oct;190(2):654-65. doi: 10.1016/0042-6822(92)90903-3.
The overall arrangement of genes in the unique central part of the bovine herpesvirus type 4 (BHV-4) genome has been deduced by analysis of short DNA sequences. Twenty-three genes conserved in at least one of the completely sequenced herpesviruses have been identified and localized. All of these genes encoded amino acid sequences with higher similarity to proteins of the gammaherpesviruses Epstein-Barr virus (EBV) and herpesvirus saimiri (HVS) than to the homologous products of the alphaherpesviruses varicella-zoster virus and herpes simplex virus type 1 or the betaherpesvirus human cytomegalovirus. The genome organization of BHV-4 had also an overall colinearity with that of the gammaherpesviruses EBV and HVS. Furthermore, the BHV-4 genes content and arrangement were more similar to those of HVS than to those of EBV, suggesting that BHV-4 and HVS are evolutionarily more closely related to each other than either are to EBV. BHV-4 DNA sequences were generally deficient in CpG dinucleotide. This CpG deficiency is characteristic of gammaherpesvirus genomes and suggests that the BHV-4 latent genome is extensively methylated. Despite several biological features similar to those of betaherpesviruses, BHV-4 displays the molecular characteristics of the representative members of the gammaherpesvirinae subfamily.
通过对短DNA序列的分析，已推断出牛疱疹病毒4型（BHV - 4）基因组独特中心部分的基因总体排列。已鉴定并定位了在至少一种完全测序的疱疹病毒中保守的23个基因。所有这些基因编码的氨基酸序列与γ疱疹病毒爱泼斯坦 - 巴尔病毒（EBV）和猴疱疹病毒（HVS）的蛋白质的相似性高于与α疱疹病毒水痘 - 带状疱疹病毒和单纯疱疹病毒1型或β疱疹病毒人巨细胞病毒的同源产物的相似性。BHV - 4的基因组组织与γ疱疹病毒EBV和HVS的基因组组织也具有总体共线性。此外，BHV - 4的基因含量和排列与HVS的更相似，而不是与EBV的相似，这表明BHV - 4和HVS在进化上彼此比它们与EBV的关系更密切。BHV - 4 DNA序列通常缺乏CpG二核苷酸。这种CpG缺乏是γ疱疹病毒基因组的特征，表明BHV - 4潜伏基因组被广泛甲基化。尽管BHV - 4具有一些与β疱疹病毒相似的生物学特征，但它显示出γ疱疹病毒亚科代表性成员的分子特征。
